DBF4 antibody (C-Term)

Catalog No. ABIN2784842

This Rabbit Polyclonal antibody specifically detects DBF4 in WB. It exhibits reactivity toward Human, Mouse, Rat, Cow, Dog, Horse, Rabbit and Guinea Pig.

Quick Overview for DBF4 antibody (C-Term) (ABIN2784842)

Target: DBF4 (DBF4 Homolog (DBF4))

Reactivity: Human, Mouse, Rat, Cow, Dog, Horse, Rabbit, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DBF4 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-DBF4 Antibody

Binding Specificity: C-Term

Purpose: Dbf4 Antibody - C-terminal region

Sequence: AELDKKRTEY LPAHEDRTCG SPVQSLLDLF QTSEEKSEFL GFTGYTENSG

Predicted Reactivity: Cow: 93%, Dog: 100%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against Dbf4. It was validated on Western Blot.

Purification: Affinity Purified

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP52246-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for DBF4

Target: DBF4 (DBF4 Homolog (DBF4))

Alternative Name: Dbf4

Background:

Background Information: Dbf4 is a regulatory subunit for CDC7 which activates its kinase activity thereby playing a central role DNA in replication and cell proliferation. Required for progression of S phase. The complex CDC7-DBF4A selectively phosphorylates MCM2 subunit at 'Ser-40' and 'Ser-53' and then is involved in regulating the initiation of DNA replication during cell cycle.

Gene Name: DBF4 homolog (S. cerevisiae)

Alternative Symbols: A, Ask, AA545217

Protein Name: Protein DBF4 homolog A

Molecular Weight: 72kDa

Gene ID: 27214

NCBI Accession: NP_001177646

UniProt: Q9QZ41

Pathways: Mitotic G1-G1/S Phases, DNA Replication