EEF1B2 antibody (Middle Region)

Catalog No. ABIN2784910

There is 1 publication for this product available. The Rabbit Polyclonal anti-EEF1B2 antibody is suitable to detect EEF1B2 in samples from Human, Mouse, Rabbit, Cow, Horse, Dog, Goat and Guinea Pig. It has been validated for WB.

Quick Overview for EEF1B2 antibody (Middle Region) (ABIN2784910)

Target: EEF1B2 (Eukaryotic Translation Elongation Factor 1 beta 2 (EEF1B2))

Reactivity: Human, Mouse, Rabbit, Cow, Horse, Dog, Goat, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EEF1B2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-EEF1B2 Antibody

Binding Specificity: Middle Region

Purpose: EEF1B2 Antibody - middle region

Sequence: VKKALGKYGP ADVEDTTGSG ATDSKDDDDI DLFGSDDEEE SEEAKRLREE

Predicted Reactivity: Cow: 86%, Dog: 93%, Goat: 86%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 85%, Rabbit: 100%

Characteristics: This is a rabbit polyclonal antibody against EEF1B2. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human EEF1B2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP52399-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-EEF1B2 antibody (ABIN2784910)

Ewing, Chu, Elisma, Li, Taylor, Climie, McBroom-Cerajewski, Robinson, OConnor, Li, Taylor, Dharsee, Ho, Heilbut, Moore, Zhang, Ornatsky, Bukhman, Ethier, Sheng, Vasilescu, Abu-Farha, Lambert, Duewel et al.: "Large-scale mapping of human protein-protein interactions by mass spectrometry. ..." in: Molecular systems biology, Vol. 3, pp. 89, (2007) (PubMed).

Target Details for EEF1B2

Target: EEF1B2 (Eukaryotic Translation Elongation Factor 1 beta 2 (EEF1B2))

Alternative Name: EEF1B2

Background:

Background Information: EEF1B2 is a translation elongation factor. The protein is a guanine nucleotide exchange factor involved in the transfer of aminoacylated tRNAs to the ribosome. This gene encodes a translation elongation factor. The protein is a guanine nucleotide exchange factor involved in the transfer of aminoacylated tRNAs to the ribosome. Alternative splicing results in three transcript variants which differ only in the 5' UTR.

Gene Name: Eukaryotic translation elongation factor 1 beta 2

Alternative Symbols: EF1B, EEF1B, EEF1B1

Protein Name: Elongation factor 1-beta

Molecular Weight: 25kDa

Gene ID: 1933

NCBI Accession: NP_066944

UniProt: P24534