EEF1B2 antibody (Middle Region)

Catalog No. ABIN2784910

Product Details anti-EEF1B2 Antibody

Target: EEF1B2 (Eukaryotic Translation Elongation Factor 1 beta 2 (EEF1B2))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rabbit, Cow, Horse, Dog, Goat, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EEF1B2 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: VKKALGKYGP ADVEDTTGSG ATDSKDDDDI DLFGSDDEEE SEEAKRLREE

Predicted Reactivity: Cow: 86%, Dog: 93%, Goat: 86%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 85%, Rabbit: 100%

Characteristics: This is a rabbit polyclonal antibody against EEF1B2. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human EEF1B2

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 225 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-EEF1B2 antibody (ABIN2784910)

Ewing, Chu, Elisma, Li, Taylor, Climie, McBroom-Cerajewski, Robinson, OConnor, Li, Taylor, Dharsee, Ho, Heilbut, Moore, Zhang, Ornatsky, Bukhman, Ethier, Sheng, Vasilescu, Abu-Farha, Lambert, Duewel et al.: "Large-scale mapping of human protein-protein interactions by mass spectrometry. ..." in: Molecular systems biology, Vol. 3, pp. 89, (2007) (PubMed).

Target Details for EEF1B2

Target: EEF1B2 (Eukaryotic Translation Elongation Factor 1 beta 2 (EEF1B2))

Alternative Name: EEF1B2 (EEF1B2 Products)

Background: EEF1B2 is a translation elongation factor. The protein is a guanine nucleotide exchange factor involved in the transfer of aminoacylated tRNAs to the ribosome. This gene encodes a translation elongation factor. The protein is a guanine nucleotide exchange factor involved in the transfer of aminoacylated tRNAs to the ribosome. Alternative splicing results in three transcript variants which differ only in the 5' UTR.
Alias Symbols: EEF1B, EEF1B1, EF1B
Protein Interaction Partner: HUWE1, EEF1G, SUMO2, UBC, EED, VARS, DNAJA1, EEF1D, FBXO6, VHL, ADRB2, NPM1, IGSF8, VCAM1, ITGA4, FN1, CSNK2A1, SMURF1, RPLP0P6, STRIP1, UBQLN2, STRN4, NUP153, PLOD3, SRSF9, RPL7A, HSPD1, GTF2F1, EEF1A1, APP, UQCRH, TPR, SNRPA, RPS2, CAND1, CUL1, CUL2, CU
Protein Size: 225

Molecular Weight: 25 kDa

Gene ID: 1933

NCBI Accession: NM_021121, NP_066944

UniProt: P24534