CAPN10 antibody (N-Term)

Catalog No. ABIN2784913

This Rabbit Polyclonal antibody specifically detects CAPN10 in WB. It exhibits reactivity toward Human, Mouse, Dog, Rabbit, Guinea Pig, Rat and Cow.

Quick Overview for CAPN10 antibody (N-Term) (ABIN2784913)

Target: CAPN10 (Calpain 10 (CAPN10))

Reactivity: Human, Mouse, Dog, Rabbit, Guinea Pig, Rat, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CAPN10 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CAPN10 Antibody

Binding Specificity: N-Term

Sequence: MRAGRGATPA RELFRDAAFP AADSSLFCDL STPLAQFRED ITWRRPQEIC

Predicted Reactivity: Cow: 92%, Dog: 100%, Guinea Pig: 93%, Human: 100%, Mouse: 93%, Rabbit: 100%, Rat: 93%

Characteristics: This is a rabbit polyclonal antibody against CAPN10. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human CAPN10

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 517 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for CAPN10

Target: CAPN10 (Calpain 10 (CAPN10))

Alternative Name: CAPN10

Background: CAPN10 is the calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.Calpains are ubiquitous, well-conserved family of calcium-dependent, cysteine proteases. The calpain proteins are heterodimers consisting of an invariant small subunit and variable large subunits. The large catalytic subunit has four domains: domain I, the N-terminal regulatory domain that is processed upon calpain activation, domain II, the protease domain, domain III, a linker domain of unknown function, and domain IV, the calmodulin-like calcium-binding domain. This gene encodes a large subunit. It is an atypical calpain in that it lacks the calmodulin-like calcium-binding domain and instead has a divergent C-terminal domain. It is similar in organization to calpains 5 and 6. This gene is associated with type 2 or non-insulin-dependent diabetes mellitus (NIDDM) and located within the NIDDM1 region. Multiple alternative transcript variants encoding different isoforms have been described for this gene.
Alias Symbols: CANP10, NIDDM1
Protein Interaction Partner: EFNB1, FLOT1, PSMA7, FANCC,
Protein Size: 517

Molecular Weight: 58 kDa

Gene ID: 11132

NCBI Accession: NM_023085, NP_075573

UniProt: Q9HC96

Pathways: Positive Regulation of Peptide Hormone Secretion