CISH antibody (N-Term)

Catalog No. ABIN2785100

The Rabbit Polyclonal anti-CISH antibody is suitable to detect CISH in samples from Human, Mouse, Rat, Pig, Cow, Horse, Dog and Rabbit. It has been validated for WB.

Quick Overview for CISH antibody (N-Term) (ABIN2785100)

Target: CISH (Cytokine Inducible SH2-Containing Protein (CISH))

Reactivity: Human, Mouse, Rat, Pig, Cow, Horse, Dog, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CISH antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CISH Antibody

Binding Specificity: N-Term

Sequence: RESGWYWGSI TASEARQHLQ KMPEGTFLVR DSTHPSYLFT LSVKTTRGPT

Predicted Reactivity: Cow: 100%, Dog: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against Cish. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide corresponding to a region of Mouse

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 257 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for CISH

Target: CISH (Cytokine Inducible SH2-Containing Protein (CISH))

Alternative Name: Cish

Background: SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. CIS is involved in the negative regulation of cytokines that signal through the JAK-STAT5 pathway such as erythropoietin, prolactin and interleukin 3 (IL3) receptor. Inhibits STAT5 trans-activation by suppressing its tyrosine phosphorylation. May be a substrate-recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.
Alias Symbols: AI385595, CIS1, Cis, F17, F23
Protein Interaction Partner: GHR, Socs2, Lepr, Epor, Prkcq, Prkcb, Prkca, Ubc, Abl1, Cul5, TCEB2, TCEB1, Stat5a,
Protein Size: 257

Molecular Weight: 28 kDa

Gene ID: 12700

NCBI Accession: NM_009895, NP_034025

UniProt: Q62225

Pathways: JAK-STAT Signaling, Response to Growth Hormone Stimulus