CISH antibody (Internal Region)

Catalog No. ABIN6260845

The Rabbit Polyclonal anti-CISH antibody (ABIN6260845) specifically detects CISH in WB and ELISA. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for CISH antibody (Internal Region) (ABIN6260845)

Target: CISH (Cytokine Inducible SH2-Containing Protein (CISH))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CISH antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-CISH Antibody

Binding Specificity: Internal Region

Specificity: CISH Antibody detects endogenous levels of total CISH.

Predicted Reactivity: Bovine,Sheep,Rabbit,Dog

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human CISH, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:1000-3000, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for CISH

Target: CISH (Cytokine Inducible SH2-Containing Protein (CISH))

Alternative Name: CISH

Background:

Description: SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. CIS is involved in the negative regulation of cytokines that signal through the JAK-STAT5 pathway such as erythropoietin, prolactin and interleukin 3 (IL3) receptor. Inhibits STAT5 trans-activation by suppressing its tyrosine phosphorylation. May be a substrate-recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins (By similarity).

Gene: CISH

Molecular Weight: 29kDa

Gene ID: 1154

UniProt: Q9NSE2

Pathways: JAK-STAT Signaling, Response to Growth Hormone Stimulus