MRPL10 antibody (N-Term)

Catalog No. ABIN2785111

Product Details anti-MRPL10 Antibody

Target: MRPL10 (Mitochondrial Ribosomal Protein L10 (MRPL10))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Dog, Guinea Pig, Rabbit, Horse, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MRPL10 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: HRRVMHFQRQ KLMAVTEYIP PKPAIHPSCL PSPPSPPQEE IGLIRLLRRE

Predicted Reactivity: Dog: 83%, Guinea Pig: 77%, Horse: 83%, Human: 100%, Mouse: 85%, Pig: 85%, Rabbit: 92%, Rat: 77%

Characteristics: This is a rabbit polyclonal antibody against MRPL10. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human MRPL10

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 261 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for MRPL10

Target: MRPL10 (Mitochondrial Ribosomal Protein L10 (MRPL10))

Alternative Name: MRPL10 (MRPL10 Products)

Synonyms: CG11488 antibody, Dmel\\CG11488 antibody, L10 antibody, 0610040E02Rik antibody, C78440 antibody, MRP-L8 antibody, Rpml8 antibody, L10MT antibody, MRP-L10 antibody, MRPL8 antibody, RPML8 antibody, L10mt antibody, zgc:112529 antibody, MRPL18 antibody, mitochondrial ribosomal protein L10 antibody, mitochondrial ribosomal protein L10 S homeolog antibody, mitochondrial 54S ribosomal protein YmL10/YmL18 antibody, mitochondrial ribosomal protein subunit L15 (predicted) antibody, mRpL10 antibody, Mrpl10 antibody, MRPL10 antibody, mrpl10.S antibody, mrpl10 antibody

Background: Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75 % protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. A pseudogene corresponding to this gene is found on chromosome 5q. Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75 % protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. A pseudogene corresponding to this gene is found on chromosome 5q.
Alias Symbols: MGC17973, MRP-L8, RPML8, L10MT, MRPL8, MRP-L10
Protein Interaction Partner: FAM9B, KLHL12, PNMA1, TCF4, REL, UBC, NPM1, MRPL1, MRPL11, MRPL41, MRPL15, MRPL13, MRPL3, MRPL12, MRPL23, SLC25A3, ILF3, ICT1, HNRNPU, ABCC2, BCS1L, APP, ABCB7, MRPL10, USP22,
Protein Size: 261

Molecular Weight: 29 kDa

Gene ID: 124995

NCBI Accession: NM_145255, NP_660298

UniProt: Q7Z7H8