MRPL10 antibody (N-Term)

Catalog No. ABIN2785111

The Rabbit Polyclonal anti-MRPL10 antibody (ABIN2785111) specifically detects MRPL10 in WB. The antibody is reactive with Human, Mouse, Rat, Dog, Guinea Pig, Rabbit, Horse and Pig samples.

Quick Overview for MRPL10 antibody (N-Term) (ABIN2785111)

Target: MRPL10 (Mitochondrial Ribosomal Protein L10 (MRPL10))

Reactivity: Human, Mouse, Rat, Dog, Guinea Pig, Rabbit, Horse, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MRPL10 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MRPL10 Antibody

Binding Specificity: N-Term

Purpose: MRPL10 Antibody - N-terminal region

Sequence: HRRVMHFQRQ KLMAVTEYIP PKPAIHPSCL PSPPSPPQEE IGLIRLLRRE

Predicted Reactivity: Dog: 83%, Guinea Pig: 77%, Horse: 83%, Human: 100%, Mouse: 85%, Pig: 85%, Rabbit: 92%, Rat: 77%

Characteristics: This is a rabbit polyclonal antibody against MRPL10. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human MRPL10

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP52830-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for MRPL10

Target: MRPL10 (Mitochondrial Ribosomal Protein L10 (MRPL10))

Alternative Name: MRPL10

Background:

Background Information: Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75 % protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. A pseudogene corresponding to this gene is found on chromosome 5q. Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75 % protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. A pseudogene corresponding to this gene is found on chromosome 5q.

Gene Name: Mitochondrial ribosomal protein L10

Alternative Symbols: L10MT, MRPL8, RPML8, MRP-L8, MRP-L10

Protein Name: 39S ribosomal protein L10, mitochondrial

Molecular Weight: 29kDa

Gene ID: 124995

NCBI Accession: NP_660298

UniProt: Q7Z7H8