MLKL antibody (N-Term)

Catalog No. ABIN2785223

Cited in 1 publication. The Rabbit Polyclonal anti-MLKL antibody (ABIN2785223) specifically detects MLKL in WB and IHC. The antibody is reactive with Human, Rat, Cow and Horse samples.

Quick Overview for MLKL antibody (N-Term) (ABIN2785223)

Target: MLKL (Mixed Lineage Kinase Domain-Like (MLKL))

Reactivity: Human, Rat, Cow, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MLKL antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-MLKL Antibody

Binding Specificity: N-Term

Purpose: MLKL Antibody - N-terminal region

Sequence: DVWKELSLLL QVEQRMPVSP ISQGASWAQE DQQDADEDRR AFQMLRRDNE

Predicted Reactivity: Cow: 85%, Horse: 91%, Human: 100%, Rat: 90%

Characteristics: This is a rabbit polyclonal antibody against MLKL. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human MLKL

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP53092-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-MLKL antibody (ABIN2785223)

Ewing, Chu, Elisma, Li, Taylor, Climie, McBroom-Cerajewski, Robinson, OConnor, Li, Taylor, Dharsee, Ho, Heilbut, Moore, Zhang, Ornatsky, Bukhman, Ethier, Sheng, Vasilescu, Abu-Farha, Lambert, Duewel et al.: "Large-scale mapping of human protein-protein interactions by mass spectrometry. ..." in: Molecular systems biology, Vol. 3, pp. 89, (2007) (PubMed).

Target Details for MLKL

Target: MLKL (Mixed Lineage Kinase Domain-Like (MLKL))

Alternative Name: MLKL

Background:

Background Information: The specific function of this protein remains unknown.

Gene Name: Mixed lineage kinase domain-like

Alternative Symbols: hMLKL

Protein Name: Mixed lineage kinase domain-like protein

Molecular Weight: 54 kDa

Gene ID: 197259

NCBI Accession: NP_689862

UniProt: Q8NB16