TTL antibody (Middle Region)

Catalog No. ABIN2785284

The Rabbit Polyclonal anti-TTL antibody is suitable to detect TTL in samples from Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit and Zebrafish (Danio rerio). It has been validated for WB.

Quick Overview for TTL antibody (Middle Region) (ABIN2785284)

Target: TTL (Tubulin tyrosine Ligase (TTL))

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TTL antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-TTL Antibody

Binding Specificity: Middle Region

Purpose: TTL Antibody - middle region

Sequence: LYREGVLRTA SEPYHVDNFQ DKTCHLTNHC IQKEYSKNYG KYEEGNEMFF

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 79%

Characteristics: This is a rabbit polyclonal antibody against TTL. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human TTL

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP53199-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for TTL

Target: TTL (Tubulin tyrosine Ligase (TTL))

Alternative Name: TTL

Background:

Background Information: TTL catalyzes the post-translational addition of a tyrosine to the C-terminal end of detyrosinated alpha-tubulin.TTL is a cytosolic enzyme involved in the posttranslational modification of alpha-tubulin (see MIM 602529). Alpha-tubulin within assembled microtubules is detyrosinated over time at the C terminus. After microtubule disassembly, TTL restores the tyrosine residues and consequently participates in a cycle of tubulin detyrosination and tyrosination (Erck et al., 2003 [PubMed 14571137]).[supplied by OMIM].

Gene Name: Tubulin tyrosine ligase

Alternative Symbols: MGC46235

Protein Name: Tubulin--tyrosine ligase

Molecular Weight: 43kDa

Gene ID: 150465

NCBI Accession: NP_714923

UniProt: Q8NG68

Pathways: Regulation of Cell Size