AP2B1 antibody (C-Term)

Catalog No. ABIN2785455

Cited in 1 publication. The Rabbit Polyclonal anti-AP2B1 antibody (ABIN2785455) specifically detects AP2B1 in WB. The antibody is reactive with Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit and Zebrafish (Danio rerio) samples.

Quick Overview for AP2B1 antibody (C-Term) (ABIN2785455)

Target: AP2B1 (Adaptor-Related Protein Complex 2, beta 1 Subunit (AP2B1))

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AP2B1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-AP2B1 Antibody

Binding Specificity: C-Term

Purpose: AP2B1 Antibody - C-terminal region

Sequence: GAVDLLGGGL DSLLGSDLGG GIGGSPAVGQ SFIPSSVPAT FAPSPTPAVV

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against AP2B1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human AP2B1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8094075

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-AP2B1 antibody (ABIN2785455)

Schmid, Ford, Burtey, Praefcke, Peak-Chew, Mills, Benmerah, McMahon: "Role of the AP2 beta-appendage hub in recruiting partners for clathrin-coated vesicle assembly." in: PLoS biology, Vol. 4, Issue 9, pp. e262, (2006) (PubMed).

Target Details for AP2B1

Target: AP2B1 (Adaptor-Related Protein Complex 2, beta 1 Subunit (AP2B1))

Alternative Name: AP2B1

Background:

Targeted Subunit: beta

Background Information: AP2B1 is one of two large chain components of the assembly protein complex 2, which serves to link clathrin to receptors in coated vesicles. AP2B1 is found on the cytoplasmic face of coated vesicles in the plasma membrane.The protein encoded by this gene is one of two large chain components of the assembly protein complex 2, which serves to link clathrin to receptors in coated vesicles. The encoded protein is found on the cytoplasmic face of coated vesicles in the plasma membrane. Two transcript variants encoding different isoforms have been found for this gene.

Gene Name: Adaptor-related protein complex 2, beta 1 subunit

Alternative Symbols: ADTB2, AP105B, CLAPB1, AP2-BETA

Protein Name: AP-2 complex subunit beta

Molecular Weight: 105kDa

Gene ID: 163

NCBI Accession: NP_001025177

UniProt: P63010

Pathways: EGFR Signaling Pathway, Neurotrophin Signaling Pathway, EGFR Downregulation