MLF1 antibody (N-Term)

Catalog No. ABIN2785560

This Rabbit Polyclonal antibody specifically detects MLF1 in WB. It exhibits reactivity toward Human, Mouse, Rat and Rabbit.It has been mentioned in 1 publication

Quick Overview for MLF1 antibody (N-Term) (ABIN2785560)

Target: MLF1 (Myeloid Leukemia Factor 1 (MLF1))

Reactivity: Human, Mouse, Rat, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MLF1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MLF1 Antibody

Binding Specificity: N-Term

Purpose: MLF1 Antibody - N-terminal region

Sequence: GRDLLSISDG RGRAHNRRGH NDGEDSLTHT DVSSFQTMDQ MVSNMRNYMQ

Predicted Reactivity: Human: 100%, Mouse: 79%, Rabbit: 90%, Rat: 79%

Characteristics: This is a rabbit polyclonal antibody against MLF1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human MLF1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8105926

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-MLF1 antibody (ABIN2785560)

Li, Wu, Jiang, Liu, Wu, Inagaki, Izawa, Mizisin, Engvall, Shelton: "Non-pathogenic protein aggregates in skeletal muscle in MLF1 transgenic mice." in: Journal of the neurological sciences, Vol. 264, Issue 1-2, pp. 77-86, (2007) (PubMed).

Target Details for MLF1

Target: MLF1 (Myeloid Leukemia Factor 1 (MLF1))

Alternative Name: MLF1

Background:

Background Information: MLF1 is involved in lineage commitment of primary hemopoietic progenitors by restricting erythroid formation and enhancing myeloid formation. It interferes with erythopoietin-induced erythroid terminal differentiation by preventing cells from exiting the cell cycle through suppression of CDKN1B/p27Kip1 levels. MLF1 suppresses RFWD2/COP1 activity via CSN3 which activates p53 and induces cell cycle arrest.It binds DNA and affects the expression of a number of genes so may function as a transcription factor in the nucleus.

Gene Name: Myeloid leukemia factor 1

Alternative Symbols: -

Protein Name: Myeloid leukemia factor 1

Molecular Weight: 29kDa

Gene ID: 4291

NCBI Accession: NP_071888

UniProt: P58340