SQSTM1 antibody (Middle Region)

Catalog No. ABIN2785619

This Rabbit Polyclonal antibody specifically detects SQSTM1 in WB and IHC. It exhibits reactivity toward Human, Mouse, Rat, Cow, Rabbit, Sheep, Horse, Dog, Guinea Pig and Zebrafish (Danio rerio).

Quick Overview for SQSTM1 antibody (Middle Region) (ABIN2785619)

Target: SQSTM1 (Sequestosome 1 (SQSTM1))

Reactivity: Human, Mouse, Rat, Cow, Rabbit, Sheep, Horse, Dog, Guinea Pig, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SQSTM1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-SQSTM1 Antibody

Binding Specificity: Middle Region

Sequence: EEQMESDNCS GGDDDWTHLS SKEVDPSTGE LQSLQMPESE GPSSLDPSQE

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Sheep: 100%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against SQSTM1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human SQSTM1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 440 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for SQSTM1

Target: SQSTM1 (Sequestosome 1 (SQSTM1))

Alternative Name: SQSTM1

Background: This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to m
Alias Symbols: A170, OSIL, PDB3, ZIP3, p60, p62, p62B
Protein Interaction Partner: HTT, BCL2, ATXN3, MAP1LC3A, tat, GABARAPL1, GABARAPL2, KEAP1, SQSTM1, UBC, TP53, TRIM63, TRIM55, MAP1LC3B, TOLLIP, ULK1, GABARAP, OPTN, SESN2, SUMO3, NBR1, EPAS1, EGLN3, CFTR, DCP2, MGRN1, NEDD8, EDEM1, LGALS3, IKBKG, TNK2, DVL2, FLNB, PSMD12, PSMD10, PSM
Protein Size: 440

Molecular Weight: 48 kDa

Gene ID: 8878

NCBI Accession: NM_003900, NP_003891

UniProt: Q13501

Pathways: NF-kappaB Signaling, Neurotrophin Signaling Pathway, Autophagy