ATG10 antibody (C-Term)

Catalog No. ABIN2785625

This Rabbit Polyclonal antibody specifically detects ATG10 in WB. It exhibits reactivity toward Human, Mouse, Rat, Dog, Rabbit, Cow, Guinea Pig and Horse.

Quick Overview for ATG10 antibody (C-Term) (ABIN2785625)

Target: ATG10 (Autophagy Related 10 (ATG10))

Reactivity: Human, Mouse, Rat, Dog, Rabbit, Cow, Guinea Pig, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATG10 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ATG10 Antibody

Binding Specificity: C-Term

Purpose: ATG10 Antibody - C-terminal region

Sequence: TPVLKNSQKI NKNVNYITSW LSIVGPVVGL NLPLSYAKAT SQDERNVP

Predicted Reactivity: Cow: 86%, Dog: 79%, Guinea Pig: 79%, Horse: 79%, Human: 100%, Mouse: 100%, Rabbit: 86%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against ATG10. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human ATG10

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8095316

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for ATG10

Target: ATG10 (Autophagy Related 10 (ATG10))

Alternative Name: ATG10

Background:

Background Information: Autophagy is a process for the bulk degradation of cytosolic compartments by lysosomes. ATG10 is an E2-like enzyme involved in 2 ubiquitin-like modifications essential for autophagosome formation: ATG12-ATG5 conjugation and modification of a soluble form of MAP-LC3 (MAP1LC3A), a homolog of yeast Apg8, to a membrane-bound form.Autophagy is a process for the bulk degradation of cytosolic compartments by lysosomes. ATG10 is an E2-like enzyme involved in 2 ubiquitin-like modifications essential for autophagosome formation: ATG12 (MIM 609608)-ATG5 (MIM 604261) conjugation and modification of a soluble form of MAP-LC3 (MAP1LC3A, MIM 601242), a homolog of yeast Apg8, to a membrane-bound form (Nemoto et al., 2003 [PubMed 12890687]).[supplied by OMIM].

Gene Name: ATG10 autophagy related 10 homolog (S. cerevisiae)

Alternative Symbols: APG10, APG10L, pp12616

Protein Name: Ubiquitin-like-conjugating enzyme ATG10

Molecular Weight: 25kDa

Gene ID: 83734

NCBI Accession: NP_113670

UniProt: Q9H0Y0

Pathways: ER-Nucleus Signaling, Autophagy