Hexokinase 2 antibody (Middle Region)

Catalog No. ABIN2785643

The Rabbit Polyclonal anti-Hexokinase 2 antibody has been validated for WB. It is suitable to detect Hexokinase 2 in samples from Human, Mouse, Rat, Dog, Rabbit, Pig, Cow, Horse and Guinea Pig.

Quick Overview for Hexokinase 2 antibody (Middle Region) (ABIN2785643)

Target: Hexokinase 2 (HK2)

Reactivity: Human, Mouse, Rat, Dog, Rabbit, Pig, Cow, Horse, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Hexokinase 2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Hexokinase 2 Antibody

Binding Specificity: Middle Region

Sequence: QRIKENKGEE RLRSTIGVDG SVYKKHPHFA KRLHKTVRRL VPGCDVRFLR

Predicted Reactivity: Cow: 79%, Dog: 100%, Guinea Pig: 92%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against HK2. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human HK2

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 917 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for Hexokinase 2

Target: Hexokinase 2 (HK2)

Alternative Name: HK2

Background: Hexokinases phosphorylate glucose to produce glucose-6-phosphate, thus committing glucose to the glycolytic pathway. HK2 (hexokinase 2) is the predominant form found in skeletal muscle. It localizes to the outer membrane of mitochondria. Expression of this protein is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells.
Alias Symbols: DKFZp686M1669, HKII, HXK2
Protein Interaction Partner: UBQLN1, MAGEA12, SUMO2, SUMO3, UBC, EGFR, rev, H2AFV, WDR61, ARIH2, PDLIM1, PYGL, NUBP1, IPO5, HEXB, FNTA, FBXO6, PARK2, UBD, ITGA4, NUDCD3, UBQLN4, SLC2A4, ELAVL1, PTGDS, ZBTB17, PRKCE, IGFBP4,
Protein Size: 917

Molecular Weight: 102 kDa

Gene ID: 3099

NCBI Accession: NM_000189, NP_000180

UniProt: P52789

Pathways: PI3K-Akt Signaling, Carbohydrate Homeostasis, Warburg Effect