LIG1 antibody (Middle Region)

Catalog No. ABIN2785647

The Rabbit Polyclonal anti-LIG1 antibody is suitable to detect LIG1 in samples from Human, Rat, Mouse, Cow, Rabbit, Dog, Horse and Pig. It has been validated for WB.

Quick Overview for LIG1 antibody (Middle Region) (ABIN2785647)

Target: LIG1 (Ligase I, DNA, ATP-Dependent (LIG1))

Reactivity: Human, Rat, Mouse, Cow, Rabbit, Dog, Horse, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LIG1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-LIG1 Antibody

Binding Specificity: Middle Region

Sequence: PSVTSFILDT EAVAWDREKK QIQPFQVLTT RKRKEVDASE IQVQVCLYAF

Predicted Reactivity: Cow: 92%, Dog: 92%, Horse: 92%, Human: 100%, Mouse: 100%, Pig: 92%, Rabbit: 85%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against LIG1. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human LIG1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 919 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for LIG1

Target: LIG1 (Ligase I, DNA, ATP-Dependent (LIG1))

Alternative Name: LIG1

Background: LIG1is DNA ligase I, with functions in DNA replication and the base excision repair process. Mutations in LIG1 that lead to DNA ligase I deficiency result in immunodeficiency and increased sensitivity to DNA-damaging agents.
Alias Symbols: MGC117397, MGC130025
Protein Interaction Partner: PCNA, UBC, PRMT3, SUMO2, RGS2, TUBB3, MRE11A, INPP1, CSNK2A1,
Protein Size: 919

Molecular Weight: 102 kDa

Gene ID: 3978

NCBI Accession: NM_000234, NP_000225

UniProt: P18858

Pathways: Telomere Maintenance, DNA Damage Repair, DNA Replication, Synthesis of DNA