Aminomethyltransferase antibody (N-Term)

Catalog No. ABIN2785650

The Rabbit Polyclonal anti-Aminomethyltransferase antibody is suitable to detect Aminomethyltransferase in samples from Human, Mouse, Rat, Dog, Cow, Guinea Pig, Horse, Rabbit and Zebrafish (Danio rerio). It has been validated for WB.

Quick Overview for Aminomethyltransferase antibody (N-Term) (ABIN2785650)

Target: Aminomethyltransferase (AMT)

Reactivity: Human, Mouse, Rat, Dog, Cow, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Aminomethyltransferase antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Aminomethyltransferase Antibody

Binding Specificity: N-Term

Purpose: AMT Antibody - N-terminal region

Sequence: QRAVSVVARL GFRLQAFPPA LCRPLSCAQE VLRRTPLYDF HLAHGGKMVA

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 92%

Characteristics: This is a rabbit polyclonal antibody against AMT. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human AMT

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP54313-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for Aminomethyltransferase

Target: Aminomethyltransferase (AMT)

Alternative Name: AMT

Background:

Background Information: The enzyme system for cleavage of glycine (glycine cleavage system, EC 2.1.2.10), which is confined to the mitochondria, is composed of 4 protein components: P protein (a pyridoxal phosphate-dependent glycine decarboxylase), H protein (a lipoic acid-containing protein), T protein (a tetrahydrofolate-requiring enzyme), and L protein (a lipoamide dehydrogenase). Glycine encephalopathy (GCE) may be due to a defect in any one of these enzymes.The enzyme system for cleavage of glycine (glycine cleavage system, EC 2.1.2.10), which is confined to the mitochondria, is composed of 4 protein components: P protein (a pyridoxal phosphate-dependent glycine decarboxylase, MIM 238300), H protein (a lipoic acid-containing protein, MIM 238330), T protein (a tetrahydrofolate-requiring enzyme), and L protein (a lipoamide dehydrogenase, MIM 238331). Glycine encephalopathy (GCE, MIM 605899) may be due to a defect in any one of these enzymes.[supplied by OMIM]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. PRIMARYREFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP 1-2102 D13811.1 1-2102 2103-2117 BC044792.1 3271-3285

Gene Name: Aminomethyltransferase

Alternative Symbols: GCE, NKH, GCST, GCVT

Protein Name: Aminomethyltransferase, mitochondrial

Molecular Weight: 44kDa

Gene ID: 275

NCBI Accession: NP_000472

UniProt: P48728