GALE antibody (N-Term)

Catalog No. ABIN2785690

The Rabbit Polyclonal anti-GALE antibody is suitable to detect GALE in samples from Human, Rat, Mouse, Dog, Cow, Pig, Rabbit and Guinea Pig. It has been validated for WB.

Quick Overview for GALE antibody (N-Term) (ABIN2785690)

Target: GALE (UDP-Galactose-4-Epimerase (GALE))

Reactivity: Human, Rat, Mouse, Dog, Cow, Pig, Rabbit, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GALE antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-GALE Antibody

Binding Specificity: N-Term

Purpose: GALE Antibody - N-terminal region

Sequence: AEKVLVTGGA GYIGSHTVLE LLEAGYLPVV IDNFHNAFRG GGSLPESLRR

Predicted Reactivity: Cow: 93%, Dog: 86%, Guinea Pig: 86%, Human: 100%, Mouse: 91%, Pig: 91%, Rabbit: 92%, Rat: 91%

Characteristics: This is a rabbit polyclonal antibody against GALE. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human GALE

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP54393-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for GALE

Target: GALE (UDP-Galactose-4-Epimerase (GALE))

Alternative Name: GALE

Background:

Background Information: GALE is an UDP-galactose-4-epimerase which catalyzes two distinct but analogous reactions: the epimerization of UDP-glucose to UDP-galactose, and the epimerization of UDP-N-acetylglucosamine to UDP-N-acetylgalactosamine. The bifunctional nature of the enzyme has the important metabolic consequence that mutant cells (or individuals) are dependent not only on exogenous galactose, but also on exogenous N-acetylgalactosamine as a necessary precursor for the synthesis of glycoproteins and glycolipids. Mutations in this gene result in epimerase-deficiency galactosemia, also referred to as galactosemia type 3, a disease characterized by liver damage, early-onset cataracts, deafness and mental retardation, with symptoms ranging from mild ('peripheral' form) to severe ('generalized' form). This gene encodes UDP-galactose-4-epimerase which catalyzes two distinct but analogous reactions: the epimerization of UDP-glucose to UDP-galactose, and the epimerization of UDP-N-acetylglucosamine to UDP-N-acetylgalactosamine. The bifunctional nature of the enzyme has the important metabolic consequence that mutant cells (or individuals) are dependent not only on exogenous galactose, but also on exogenous N-acetylgalactosamine as a necessary precursor for the synthesis of glycoproteins and glycolipids. Mutations in this gene result in epimerase-deficiency galactosemia, also referred to as galactosemia type 3, a disease characterized by liver damage, early-onset cataracts, deafness and mental retardation, with symptoms ranging from mild ('peripheral' form) to severe ('generalized' form). Multiple alternatively spliced transcripts encoding the same protein have been identified.

Gene Name: UDP-galactose-4-epimerase

Alternative Symbols: SDR1E1

Protein Name: UDP-glucose 4-epimerase

Molecular Weight: 38kDa

Gene ID: 2582

NCBI Accession: NP_001008217

UniProt: Q14376

Pathways: Response to Water Deprivation, Cellular Glucan Metabolic Process