KPNA1 antibody (N-Term)

Catalog No. ABIN785702

The Rabbit Polyclonal anti-KPNA1 antibody is suitable to detect KPNA1 in samples from Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio) and Saccharomyces cerevisiae. It has been validated for WB.

Quick Overview for KPNA1 antibody (N-Term) (ABIN785702)

Target: KPNA1 (Karyopherin alpha 1 (Importin alpha 5) (KPNA1))

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio), Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KPNA1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-KPNA1 Antibody

Binding Specificity: N-Term

Purpose: KPNA1 Antibody - N-terminal region

Sequence: NMEMAPGGVI TSDMIEMIFS KSPEQQLSAT QKFRKLLSKE PNPPIDEVIS

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Yeast: 77%, Zebrafish: 85%

Characteristics: This is a rabbit polyclonal antibody against KPNA1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human KPNA1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP54671-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for KPNA1

Target: KPNA1 (Karyopherin alpha 1 (Importin alpha 5) (KPNA1))

Alternative Name: KPNA1

Background:

Targeted Subunit: alpha-1

Background Information: Recombination activating proteins RAG1 and RAG2 regulate and mediate V(D)J recombination, the process by which genes for immunoglobulins and T-cell receptors are generated. Several other ubiquitously expressed proteins are thought to be recruited in the recombination process. Among these are the genes affected in severe combined immune deficiency and genes involved in ds-DNA break repair. KPNA1 interacts with RAG1 and may play a role in V(D)J recombination.Recombination activating proteins RAG1 and RAG2 regulate and mediate V(D)J recombination, the process by which genes for immunoglobulins and T-cell receptors are generated. Several other ubiquitously expressed proteins are thought to be recruited in the recombination process. Among these are the genes affected in severe combined immune deficiency and genes involved in ds-DNA break repair. The protein encoded by this gene interacts with RAG1 and may play a role in V(D)J recombination. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Gene Name: Karyopherin alpha 1 (importin alpha 5)

Alternative Symbols: RCH2, SRP1, IPOA5, NPI-1

Protein Name: Importin subunit alpha-1

Molecular Weight: 60kDa

Gene ID: 3836

NCBI Accession: NP_002255

UniProt: P52294

Pathways: M Phase, Protein targeting to Nucleus