Galectin 3 antibody (N-Term)

Catalog No. ABIN2785832

There is 1 publication for this product available. The Rabbit Polyclonal anti-Galectin 3 antibody is suitable to detect Galectin 3 in samples from Human, Mouse, Rat, Rabbit, Pig, Cow, Dog, Guinea Pig and Horse. It has been validated for WB and IHC.

Quick Overview for Galectin 3 antibody (N-Term) (ABIN2785832)

Target: Galectin 3 (LGALS3)

Reactivity: Human, Mouse, Rat, Rabbit, Pig, Cow, Dog, Guinea Pig, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Galectin 3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Galectin 3 Antibody

Binding Specificity: N-Term

Purpose: LGALS3 Antibody - N-terminal region

Sequence: GASYPGAYPG QAPPGAYPGQ APPGAYPGAP GAYPGAPAPG VYPGPPSGPG

Predicted Reactivity: Cow: 93%, Dog: 93%, Guinea Pig: 92%, Horse: 92%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 92%, Rat: 93%

Characteristics: This is a rabbit polyclonal antibody against LGALS3. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human LGALS3

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP54688-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-Galectin 3 antibody (ABIN2785832)

Guha, Bandyopadhyaya, Polumuri, Chumsri, Gade, Kalvakolanu, Ahmed et al.: "Nicotine promotes apoptosis resistance of breast cancer cells and enrichment of side population cells with cancer stem cell-like properties via a signaling cascade involving galectin-3, α9 nicotinic ..." in: Breast cancer research and treatment, Vol. 145, Issue 1, pp. 5-22, (2014) (PubMed).

Target Details for Galectin 3

Target: Galectin 3 (LGALS3)

Alternative Name: LGALS3

Background:

Background Information: This gene encodes a member of the galectin family of carbohydrate binding proteins. Members of this protein family have an affinity for beta-galactosides. The encoded protein is characterized by an N-terminal proline-rich tandem repeat domain and a single C-terminal carbohydrate recognition domain. This protein can self-associate through the N-terminal domain allowing it to bind to multivalent saccharide ligands. This protein localizes to the extracellular matrix, the cytoplasm and the nucleus. This protein plays a role in numerous cellular functions including apoptosis, innate immunity, cell adhesion and T-cell regulation. The protein exhibits antimicrobial activity against bacteria and fungi. Alternate splicing results in multiple transcript variants.

Gene Name: galectin 3

Alternative Symbols: L31, GAL3, MAC2, CBP35, GALBP, GALIG, LGALS2

Protein Name: galectin-3

Molecular Weight: 26kDa

Gene ID: 3958

NCBI Accession: NP_002297

UniProt: P17931

Pathways: RTK Signaling