LIF antibody (N-Term)

Catalog No. ABIN2785833

Product Details anti-LIF Antibody

Target: LIF (Leukemia Inhibitory Factor (LIF))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Cow, Dog, Sheep, Guinea Pig, Horse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LIF antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: KVLAAGVVPL LLVLHWKHGA GSPLPITPVN ATCAIRHPCH NNLMNQIRSQ

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 86%, Rat: 100%, Sheep: 86%

Characteristics: This is a rabbit polyclonal antibody against LIF. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human LIF

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 202 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-LIF antibody (ABIN2785833)

Muntz, Malpass, McGonigle, Robertson, Weiden: "Phase 2 study of intraperitoneal topotecan as consolidation chemotherapy in ovarian and primary peritoneal carcinoma." in: Cancer, Vol. 113, Issue 3, pp. 490-6, (2008) (PubMed).

Target Details for LIF

Target: LIF (Leukemia Inhibitory Factor (LIF))

Alternative Name: LIF (LIF Products)

Synonyms: CDF antibody, DIA antibody, HILDA antibody, MLPLI antibody, LIF, interleukin 6 family cytokine antibody, leukemia inhibitory factor antibody, LIF antibody, Lif antibody

Background: LIF is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface.Leukaemia inhibitory factor is a cytokine that induces macrophage differentiation. Neurotransmitters and neuropeptides, well known for their role in the communication between neurons, are also capable of activating monocytes and macrophages and inducing chemotaxis in immune cells. LIF signals through different receptors and transcription factors. LIF in conjunction with BMP2 acts in synergy on primary fetal neural progenitor cells to induce astrocytes. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: CDF, D-FACTOR, HILDA, DIA
Protein Interaction Partner: IL6ST, LIFR,
Protein Size: 202

Molecular Weight: 22 kDa

Gene ID: 3976

NCBI Accession: NM_002309, NP_002300

UniProt: P15018

Pathways: JAK-STAT Signaling, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Stem Cell Maintenance, Growth Factor Binding