IDH3G antibody (C-Term)

Catalog No. ABIN2785851

The Rabbit Polyclonal anti-IDH3G antibody is suitable to detect IDH3G in samples from Human, Mouse, Rat, Cow, Dog, Horse, Goat, Guinea Pig, Rabbit, Saccharomyces cerevisiae and Zebrafish (Danio rerio). It has been validated for WB.

Quick Overview for IDH3G antibody (C-Term) (ABIN2785851)

Target: IDH3G (Isocitrate Dehydrogenase 3 (NAD+) gamma (IDH3G))

Reactivity: Human, Mouse, Rat, Cow, Dog, Horse, Goat, Guinea Pig, Rabbit, Saccharomyces cerevisiae, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IDH3G antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-IDH3G Antibody

Binding Specificity: C-Term

Purpose: IDH3G Antibody - C-terminal region

Sequence: YMTRHNNLDL VIIREQTEGE YSSLEHEVRP QKLGEGKDED GREVELLVSL

Predicted Reactivity: Cow: 100%, Dog: 100%, Goat: 93%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Yeast: 93%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against IDH3B. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C-terminal region of human IDH3G

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP54721-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for IDH3G

Target: IDH3G (Isocitrate Dehydrogenase 3 (NAD+) gamma (IDH3G))

Alternative Name: IDH3G

Background:

Background Information: Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit. The protein encoded by this gene is the gamma subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase. This gene is a candidate gene for periventricular heterotopia. Several alternatively spliced transcript variants of this gene have been described, but only some of their full length natures have been determined.

Gene Name: isocitrate dehydrogenase 3 (NAD+) gamma

Alternative Symbols: H-IDHG

Protein Name: isocitrate dehydrogenase [NAD] subunit gamma, mitochondrial

Molecular Weight: 22kDa

Gene ID: 3421

NCBI Accession: NP_004126

UniProt: P51553