PHGDH antibody (Middle Region)

Catalog No. ABIN2785892

This Rabbit Polyclonal antibody specifically detects PHGDH in WB. It exhibits reactivity toward Human, Mouse, Rat, Horse, Pig, Rabbit, Dog, Cow, Guinea Pig and Zebrafish (Danio rerio).

Quick Overview for PHGDH antibody (Middle Region) (ABIN2785892)

Target: PHGDH (phosphoglycerate Dehydrogenase (PHGDH))

Reactivity: Human, Mouse, Rat, Horse, Pig, Rabbit, Dog, Cow, Guinea Pig, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PHGDH antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PHGDH Antibody

Binding Specificity: Middle Region

Purpose: PHGDH Antibody - middle region

Sequence: CAGAALDVFT EEPPRDRALV DHENVISCPH LGASTKEAQS RCGEEIAVQF

Predicted Reactivity: Cow: 93%, Dog: 92%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 79%

Characteristics: This is a rabbit polyclonal antibody against PHGDH. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human PHGDH

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP54801-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for PHGDH

Target: PHGDH (phosphoglycerate Dehydrogenase (PHGDH))

Alternative Name: PHGDH

Background:

Background Information: 3-Phosphoglycerate dehydrogenase (PHGDH) catalyzes the transition of 3-phosphoglycerate into 3-phosphohydroxypyruvate, which is the first and rate-limiting step in the phosphorylated pathway of serine biosynthesis, using NAD+/NADH as a cofactor.3-Phosphoglycerate dehydrogenase (PHGDH, EC 1.1.1.95) catalyzes the transition of 3-phosphoglycerate into 3-phosphohydroxypyruvate, which is the first and rate-limiting step in the phosphorylated pathway of serine biosynthesis, using NAD+/NADH as a cofactor.[supplied by OMIM].

Gene Name: Phosphoglycerate dehydrogenase

Alternative Symbols: NLS, PDG, PGD, NLS1, PGAD, PGDH, SERA, 3PGDH, 3-PGDH, PHGDHD, HEL-S-113

Protein Name: D-3-phosphoglycerate dehydrogenase

Molecular Weight: 57 kDa

Gene ID: 26227

NCBI Accession: NP_006614

UniProt: O43175

Pathways: Metabolism of Steroid Hormones and Vitamin D, Warburg Effect