RABGEF1 antibody (N-Term)

Catalog No. ABIN2786039

The Rabbit Polyclonal anti-RABGEF1 antibody (ABIN2786039) specifically detects RABGEF1 in WB. The antibody is reactive with Human, Mouse, Rat, Cow, Dog, Goat, Horse, Zebrafish (Danio rerio), Rabbit and Guinea Pig samples.

Quick Overview for RABGEF1 antibody (N-Term) (ABIN2786039)

Target: RABGEF1 (RAB Guanine Nucleotide Exchange Factor (GEF) 1 (RABGEF1))

Reactivity: Human, Mouse, Rat, Cow, Dog, Goat, Horse, Zebrafish (Danio rerio), Rabbit, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RABGEF1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-RABGEF1 Antibody

Binding Specificity: N-Term

Purpose: RABGEF1 Antibody - N-terminal region

Sequence: MSLKSERRGI HVDQSDLLCK KGCGYYGNPA WQGFCSKCWR EEYHKARQKQ

Predicted Reactivity: Cow: 100%, Dog: 100%, Goat: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 86%

Characteristics: This is a rabbit polyclonal antibody against RABGEF1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human RABGEF1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP55073-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for RABGEF1

Target: RABGEF1 (RAB Guanine Nucleotide Exchange Factor (GEF) 1 (RABGEF1))

Alternative Name: RABGEF1

Background:

Background Information: RABGEF1 forms a complex with rabaptin-5 (RABPT5, MIM 603616) that is required for endocytic membrane fusion, and it serves as a specific guanine nucleotide exchange factor (GEF) for RAB5 (RAB5A, MIM 179512) (Horiuchi et al., 1997 [PubMed 9323142]).

Gene Name: RAB guanine nucleotide exchange factor (GEF) 1

Alternative Symbols: RAP1, RABEX5, rabex-5

Protein Name: Rab5 GDP/GTP exchange factor

Molecular Weight: 57kDa

Gene ID: 27342

NCBI Accession: NP_055319

UniProt: A8K3R3

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process