Glutaminase antibody (C-Term)

Catalog No. ABIN503647

This Rabbit Polyclonal antibody specifically detects Glutaminase in WB. It exhibits reactivity toward Human, Mouse, Rat, Dog, Guinea Pig, Horse and Rabbit.It has been mentioned in 1 publication

Quick Overview for Glutaminase antibody (C-Term) (ABIN503647)

Target: Glutaminase (GLS)

Reactivity: Human, Mouse, Rat, Dog, Guinea Pig, Horse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Glutaminase antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Glutaminase Antibody

Binding Specificity: C-Term

Purpose: GLS Antibody - C-terminal region

Sequence: VNPFPKDRWN NTPMDEALHF GHHDVFKILQ EYQVQYTPQG DSDNGKENQT

Predicted Reactivity: Dog: 86%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 86%, Rabbit: 100%, Rat: 93%

Characteristics: This is a rabbit polyclonal antibody against GLS. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the c terminal region of human GLS

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP55098-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-Glutaminase antibody (ABIN503647)

Sahai: "Glutaminase in human platelets." in: Clinica chimica acta; international journal of clinical chemistry, Vol. 127, Issue 2, pp. 197-203, (1983) (PubMed).

Target Details for Glutaminase

Target: Glutaminase (GLS)

Alternative Name: GLS

Background:

Background Information: Sahai (1983) [PubMed 6825316] demonstrated phosphate-activated glutaminase (EC 3.5.1.2) in human platelets. It is the major enzyme yielding glutamate from glutamine. Significance of the enzyme derives from its possible implication in behavior disturbances

Gene Name: Glutaminase

Alternative Symbols: GAC, GAM, KGA, GLS1, AAD20, DEE71, GDPAG, CASGID, EIEE71

Protein Name: Glutaminase kidney isoform, mitochondrial

Molecular Weight: 73kDa

Gene ID: 2744

NCBI Accession: NP_055720

UniProt: O94925

Pathways: Feeding Behaviour, Dicarboxylic Acid Transport, Warburg Effect