FBXO28 antibody (Middle Region)

Catalog No. ABIN2786088

The Rabbit Polyclonal anti-FBXO28 antibody (ABIN2786088) specifically detects FBXO28 in WB. The antibody is reactive with Human, Mouse, Rat, Cow, Dog, Horse, Rabbit, Guinea Pig, Saccharomyces cerevisiae and Zebrafish (Danio rerio) samples.

Quick Overview for FBXO28 antibody (Middle Region) (ABIN2786088)

Target: FBXO28 (F-Box Protein 28 (FBXO28))

Reactivity: Human, Mouse, Rat, Cow, Dog, Horse, Rabbit, Guinea Pig, Saccharomyces cerevisiae, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FBXO28 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-FBXO28 Antibody

Binding Specificity: Middle Region

Purpose: FBXO28 Antibody - middle region

Sequence: KVQEQQKQLQ DQDQKLLEQT QIIGEQNARL AELERKLREV MESAVGNSSG

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Yeast: 75%, Zebrafish: 91%

Characteristics: This is a rabbit polyclonal antibody against FBXO28. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human FBXO28

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP55191-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for FBXO28

Target: FBXO28 (F-Box Protein 28 (FBXO28))

Alternative Name: FBXO28

Background:

Background Information: Members of the F-box protein family, such as FBXO28, are characterized by an approximately 40-amino acid F-box motif. SCF complexes, formed by SKP1, cullin, and F-box proteins, act as protein-ubiquitin ligases. F-box proteins interact with SKP1 through the F box, and they interact with ubiquitination targets through other protein interaction domains.Members of the F-box protein family, such as FBXO28, are characterized by an approximately 40-amino acid F-box motif. SCF complexes, formed by SKP1 (MIM 601434), cullin (see CUL1, MIM 603134), and F-box proteins, act as protein-ubiquitin ligases. F-box proteins interact with SKP1 through the F box, and they interact with ubiquitination targets through other protein interaction domains (Jin et al., 2004 [PubMed 15520277]).[supplied by OMIM].

Gene Name: F-box protein 28

Alternative Symbols: Fbx28, CENP-30

Protein Name: F-box only protein 28

Molecular Weight: 41kDa

Gene ID: 23219

NCBI Accession: NP_055991

UniProt: Q9NVF7