ALKBH3 antibody (Middle Region)

Catalog No. ABIN2786204

This Rabbit Polyclonal antibody specifically detects ALKBH3 in WB. It exhibits reactivity toward Human, Mouse, Rat, Horse, Rabbit, Cow, Dog, Guinea Pig and Pigand has been mentioned in 1 publication.

Quick Overview for ALKBH3 antibody (Middle Region) (ABIN2786204)

Target: ALKBH3 (AlkB, Alkylation Repair Homolog 3 (ALKBH3))

Reactivity: Human, Mouse, Rat, Horse, Rabbit, Cow, Dog, Guinea Pig, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ALKBH3 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ALKBH3 Antibody

Binding Specificity: Middle Region

Sequence: EMRKKPPPEE NGDYTYVERV KIPLDHGTLL IMEGATQADW QHRVPKEYHS

Predicted Reactivity: Cow: 93%, Dog: 93%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 86%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against ALKBH3. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human ALKBH3

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 286 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-ALKBH3 antibody (ABIN2786204)

Sundheim, Vågbø, Bjørås, Sousa, Talstad, Aas, Drabløs, Krokan, Tainer, Slupphaug: "Human ABH3 structure and key residues for oxidative demethylation to reverse DNA/RNA damage." in: The EMBO journal, Vol. 25, Issue 14, pp. 3389-97, (2006) (PubMed).

Target Details for ALKBH3

Target: ALKBH3 (AlkB, Alkylation Repair Homolog 3 (ALKBH3))

Alternative Name: ALKBH3

Background: The Escherichia coli AlkB protein protects against the cytotoxicity of methylating agents by repair of the specific DNA lesions generated in single-stranded DNA. ALKBH2 (MIM 610602) and ALKBH3 are E. coli AlkB homologs that catalyze the removal of 1-methyladenine and 3-methylcytosine.The Escherichia coli AlkB protein protects against the cytotoxicity of methylating agents by repair of the specific DNA lesions generated in single-stranded DNA. ALKBH2 (MIM 610602) and ALKBH3 are E. coli AlkB homologs that catalyze the removal of 1-methyladenine and 3-methylcytosine (Duncan et al., 2002 [PubMed 12486230]).[supplied by OMIM]. Sequence Note: removed 1 base from the 5' end that did not align to the reference genome assembly. PRIMARYREFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP 1-1519 AB042029.1 2-1520
Alias Symbols: ABH3, DEPC-1, DEPC1, MGC118790, MGC118792, MGC118793, PCA1
Protein Interaction Partner: AK8, LNX1, GLRX3, IKZF1, GOLGA2, SRPK2, SRPK1,
Protein Size: 286

Molecular Weight: 33 kDa

Gene ID: 221120

NCBI Accession: NM_139178, NP_631917

UniProt: Q96Q83

Pathways: DNA Damage Repair