PSMB2 antibody (Middle Region)

Catalog No. ABIN2786716

This Rabbit Polyclonal antibody specifically detects PSMB2 in WB. It exhibits reactivity toward Human, Mouse, Rat, Cow, Dog, Guinea Pig, Rabbit, Horse and Zebrafish (Danio rerio).

Quick Overview for PSMB2 antibody (Middle Region) (ABIN2786716)

Target: PSMB2 (Proteasome (Prosome, Macropain) Subunit, beta Type 2 (PSMB2))

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Rabbit, Horse, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PSMB2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PSMB2 Antibody

Binding Specificity: Middle Region

Purpose: PSMB2 Antibody - middle region

Sequence: LDRYYTPTIS RERAVELLRK CLEELQKRFI LNLPTFSVRI IDKNGIHDLD

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 93%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 86%

Characteristics: This is a rabbit polyclonal antibody against PSMB2. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human PSMB2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP56462-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for PSMB2

Target: PSMB2 (Proteasome (Prosome, Macropain) Subunit, beta Type 2 (PSMB2))

Alternative Name: PSMB2

Background:

Targeted Subunit: beta type-2

Background Information: The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits, 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. PSMB2 is a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits, 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Gene Name: Proteasome (prosome, macropain) subunit, beta type, 2

Alternative Symbols: HC7-I

Protein Name: Proteasome subunit beta type-2

Molecular Weight: 23kDa

Gene ID: 5690

NCBI Accession: NP_002785

UniProt: P49721

Pathways: Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA