RPA1 antibody (Middle Region)

Catalog No. ABIN2786755

The Rabbit Polyclonal anti-RPA1 antibody (ABIN2786755) specifically detects RPA1 in WB. The antibody is reactive with Human, Mouse, Rat, Rabbit, Guinea Pig, Cow, Dog, Horse, Sheep, Saccharomyces cerevisiae, Pig and Zebrafish (Danio rerio) samples.

Quick Overview for RPA1 antibody (Middle Region) (ABIN2786755)

Target: RPA1 (Replication Protein A1, 70kDa (RPA1))

Reactivity: Human, Mouse, Rat, Rabbit, Guinea Pig, Cow, Dog, Horse, Sheep, Saccharomyces cerevisiae, Pig, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RPA1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-RPA1 Antibody

Binding Specificity: Middle Region

Purpose: RPA1 Antibody - middle region

Sequence: SRGEGKLFSL ELVDESGEIR ATAFNEQVDK FFPLIEVNKV YYFSKGTLKI

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 100%, Rat: 100%, Sheep: 100%, Yeast: 93%, Zebrafish: 92%

Characteristics: This is a rabbit polyclonal antibody against RPA1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human RPA1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP56525-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for RPA1

Target: RPA1 (Replication Protein A1, 70kDa (RPA1))

Alternative Name: RPA1

Background:

Background Information: RPA1 plays an essential role in several cellular processes in DNA metabolism including replication, recombination and DNA repair. RPA1 binds and subsequently stabilizes single-stranded DNA intermediates and thus prevents complementary DNA from reannealing

Gene Name: Replication protein A1, 70 kDa

Alternative Symbols: HSSB, RF-A, RP-A, REPA1, RPA70, MST075

Protein Name: Replication protein A 70 kDa DNA-binding subunit

Molecular Weight: 68kDa

Gene ID: 6117

NCBI Accession: NP_002936

UniProt: P27694

Pathways: Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA