RAB5 antibody (Middle Region)

Catalog No. ABIN2786773

This Rabbit Polyclonal antibody specifically detects RAB5 in WB and IHC. It exhibits reactivity toward Human, Mouse, Rat, Cow, Dog, Sheep, Horse, Guinea Pig and Rabbit.It has been mentioned in 1 publication

Quick Overview for RAB5 antibody (Middle Region) (ABIN2786773)

Target: RAB5 (RAB5A) (RAB5A, Member RAS Oncogene Family (RAB5A))

Reactivity: Human, Mouse, Rat, Cow, Dog, Sheep, Horse, Guinea Pig, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAB5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-RAB5 Antibody

Binding Specificity: Middle Region

Purpose: RAB5A Antibody - middle region

Sequence: KTSMNVNEIF MAIAKKLPKN EPQNPGANSA RGRGVDLTEP TQPTRNQCCS

Predicted Reactivity: Cow: 93%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 93%, Rat: 100%, Sheep: 79%

Characteristics: This is a rabbit polyclonal antibody against RAB5A. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human RAB5A

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP56563-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-RAB5 antibody (ABIN2786773)

Jong, Wu, Li, Sun, Fabbri, Wayne, Seeger: "Large-scale isolation and cytotoxicity of extracellular vesicles derived from activated human natural killer cells." in: Journal of extracellular vesicles, Vol. 6, Issue 1, pp. 1294368, (2017) (PubMed).

Target Details for RAB5

Target: RAB5 (RAB5A) (RAB5A, Member RAS Oncogene Family (RAB5A))

Alternative Name: RAB5A

Background:

Background Information: RAB5A is required for the fusion of plasma membranes and early endosomes.

Gene Name: RAB5A, member RAS oncogene family

Alternative Symbols: RAB5

Protein Name: Ras-related protein Rab-5A

Molecular Weight: 24kDa

Gene ID: 5868

NCBI Accession: NP_004153

UniProt: P20339

Pathways: Smooth Muscle Cell Migration, Regulation of long-term Neuronal Synaptic Plasticity