NDUFS1 antibody (Middle Region)

Catalog No. ABIN2786794

This Rabbit Polyclonal antibody specifically detects NDUFS1 in WB. It exhibits reactivity toward Human, Rat, Mouse, Cow, Rabbit, Horse, Dog, Goat, Guinea Pig and Zebrafish (Danio rerio).

Quick Overview for NDUFS1 antibody (Middle Region) (ABIN2786794)

Target: NDUFS1 (NADH Dehydrogenase (Ubiquinone) Fe-S Protein 1, 75kDa (NADH-Coenzyme Q Reductase) (NDUFS1))

Reactivity: Human, Rat, Mouse, Cow, Rabbit, Horse, Dog, Goat, Guinea Pig, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NDUFS1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-NDUFS1 Antibody

Binding Specificity: Middle Region

Sequence: TPPGLAREDW KIIRALSEIA GMTLPYDTLD QVRNRLEEVS PNLVRYDDIE

Predicted Reactivity: Cow: 100%, Dog: 93%, Goat: 100%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 79%

Characteristics: This is a rabbit polyclonal antibody against NDUFS1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human NDUFS1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 727 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for NDUFS1

Target: NDUFS1 (NADH Dehydrogenase (Ubiquinone) Fe-S Protein 1, 75kDa (NADH-Coenzyme Q Reductase) (NDUFS1))

Alternative Name: NDUFS1

Background: The protein encoded by this gene belongs to the complex I 75 kDa subunit family. Mammalian complex I is composed of 45 different subunits. It locates at the mitochondrial inner membrane. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. This protein is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized. Mutations in this gene are associated with complex I deficiency.
Alias Symbols: CI-75Kd, MGC26839, PRO1304, CI-75k
Protein Interaction Partner: SUMO2, HSFY1, UBC, MDM2, RNF2, KDM1A, FBXO6, VCAM1, BTK, Htt, NDUFS7, RHOT2, NDUFA12, TMEM126B, MRPL13, STX12, SEC24C, SDHB, RPL27, RAB5B, OPA1, NDUFV3, NDUFV2, NDUFS8, NDUFV1, NDUFS2, NDUFB10, NDUFB9, NDUFB5, NDUFA9, CPT1A, ATP5C1, ATP5B, CUL3, SIRT7, MY
Protein Size: 727

Molecular Weight: 77 kDa

Gene ID: 4719

NCBI Accession: NM_005006, NP_004997

UniProt: P28331