Nitrilase 1 antibody (N-Term)

Catalog No. ABIN2786816

This Rabbit Polyclonal antibody specifically detects Nitrilase 1 in WB. It exhibits reactivity toward Human, Rat, Dog, Guinea Pig, Cow, Rabbit, Horse and Pig.

Quick Overview for Nitrilase 1 antibody (N-Term) (ABIN2786816)

Target: Nitrilase 1 (NIT1)

Reactivity: Human, Rat, Dog, Guinea Pig, Cow, Rabbit, Horse, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nitrilase 1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Nitrilase 1 Antibody

Binding Specificity: N-Term

Purpose: NIT1 Antibody - N-terminal region

Sequence: VREAARLGAC LAFLPEAFDF IARDPAETLH LSEPLGGKLL EEYTQLAREC

Predicted Reactivity: Cow: 86%, Dog: 86%, Guinea Pig: 79%, Horse: 79%, Human: 100%, Pig: 93%, Rabbit: 79%, Rat: 79%

Characteristics: This is a rabbit polyclonal antibody against NIT1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human NIT1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP56647-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for Nitrilase 1

Target: Nitrilase 1 (NIT1)

Alternative Name: NIT1

Background:

Background Information: NIT1 play a role in cell growth and apoptosis: loss of expression promotes cell growth and resistance to DNA damage stress. NIT1 has tumor suppressor properties that enhances the apoptotic responsiveness in cancer cells, this effect is additive to the tum

Gene Name: Nitrilase 1

Alternative Symbols: MGC57670

Protein Name: Nitrilase homolog 1

Molecular Weight: 36kDa

Gene ID: 4817

NCBI Accession: NP_005591

UniProt: Q86X76