MED31 antibody (N-Term)

Catalog No. ABIN2786919

The Rabbit Polyclonal anti-MED31 antibody is suitable to detect MED31 in samples from Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse and Rabbit. It has been validated for WB and ChIP.

Quick Overview for MED31 antibody (N-Term) (ABIN2786919)

Target: MED31 (Mediator Complex Subunit 31 (MED31))

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MED31 antibody is un-conjugated

Application: Western Blotting (WB), Chromatin Immunoprecipitation (ChIP)

Product Details anti-MED31 Antibody

Binding Specificity: N-Term

Purpose: MED31 Antibody - N-terminal region

Sequence: MAAAVAMETD DAGNRLRFQL ELEFVQCLAN PNYLNFLAQR GYFKDKAFVN

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against MED31. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human MED31

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP56820-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for MED31

Target: MED31 (Mediator Complex Subunit 31 (MED31))

Alternative Name: MED31

Background:

Targeted Subunit: 31

Background Information: MED31 is the component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal

Gene Name: Mediator complex subunit 31

Alternative Symbols: Soh1, CGI-125, 3110004H13Rik

Protein Name: Mediator of RNA polymerase II transcription subunit 31

Molecular Weight: 16kDa

Gene ID: 51003

NCBI Accession: NP_057144

UniProt: Q9Y3C7

Pathways: Regulation of Lipid Metabolism by PPARalpha