ARFGAP1 antibody (Middle Region)

Catalog No. ABIN2787084

This anti-ARFGAP1 antibody is a Rabbit Polyclonal antibody detecting ARFGAP1 in WB. Suitable for Human, Mouse, Rat, Rabbit, Dog, Cow and Guinea Pig.

Quick Overview for ARFGAP1 antibody (Middle Region) (ABIN2787084)

Target: ARFGAP1 (ADP-Ribosylation Factor GTPase Activating Protein 1 (ARFGAP1))

Reactivity: Human, Mouse, Rat, Rabbit, Dog, Cow, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ARFGAP1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ARFGAP1 Antibody

Binding Specificity: Middle Region

Sequence: GQPQSVTASS DKAFEDWLND DLGSYQGAQG NRYVGFGNTP PPQKKEDDFL

Predicted Reactivity: Cow: 93%, Dog: 93%, Guinea Pig: 93%, Human: 100%, Mouse: 93%, Rabbit: 93%, Rat: 93%

Characteristics: This is a rabbit polyclonal antibody against ARFGAP1. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human ARFGAP1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 406 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for ARFGAP1

Target: ARFGAP1 (ADP-Ribosylation Factor GTPase Activating Protein 1 (ARFGAP1))

Alternative Name: ARFGAP1

Background: The protein encoded by this gene is a GTPase-activating protein (GAP) which associates with the Golgi apparatus and which interacts with ADP-ribosylation factor 1 (ARF1). The encoded protein promotes hydrolysis of ARF1-bound GTP and is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is required for the fusion of these vesicles with target compartments. The activity of this protein is stimulated by phosphoinosides and inhibited by phosphatidylcholine. Two transcript variants encoding different isoforms have been found for this gene.
Alias Symbols: ARF1GAP, HRIHFB2281, MGC39924
Protein Interaction Partner: KRTAP10-9, KRTAP9-2, KRTAP3-2, GABARAPL2, GRN, FOS, TRIM23, UBC, RPL22, HK1, RAD52, RPL11, RFC3, PRKACB, APOE, APOC3, APOB, ASB10, RASIP1, RAB23, COPS5, MED27, REEP5, APP, LRRK2, MAP1LC3B, TK1, SMN1, TMED2, CYTH2, COPA, CSNK1D, RGS2, KDELR1, ARF1,
Protein Size: 406

Molecular Weight: 45 kDa

Gene ID: 55738

NCBI Accession: NM_018209, NP_060679

UniProt: Q8N6T3

Pathways: p53 Signaling, ER-Nucleus Signaling, Unfolded Protein Response