ARFGAP1 antibody (Internal Region)

Catalog No. ABIN6257929

Product Details anti-ARFGAP1 Antibody

Target: ARFGAP1 (ADP-Ribosylation Factor GTPase Activating Protein 1 (ARFGAP1))

Binding Specificity: Internal Region

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ARFGAP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: ARFGAP1 Antibody detects endogenous levels of total ARFGAP1.

Predicted Reactivity: Rabbit

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human ARFGAP1, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:1000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for ARFGAP1

Target: ARFGAP1 (ADP-Ribosylation Factor GTPase Activating Protein 1 (ARFGAP1))

Alternative Name: ARFGAP1 (ARFGAP1 Products)

Synonyms: ARF1GAP antibody, HRIHFB2281 antibody, AI115377 antibody, Arf1gap antibody, Arf GAP1 antibody, arf1gap antibody, atfb1 antibody, atfb3 antibody, jlns1 antibody, k(v)7.1 antibody, kcna8 antibody, kcna9 antibody, kv1.9 antibody, kv7.1 antibody, kvlqt1 antibody, lqt antibody, lqt1 antibody, rws antibody, sqt2 antibody, wrs antibody, ARFGAP3 antibody, ADP ribosylation factor GTPase activating protein 1 antibody, ADP-ribosylation factor GTPase activating protein 1 antibody, ADP ribosylation factor GTPase activating protein 1 S homeolog antibody, putative ADP-ribosylation factor GTPase activating protein 1 antibody, ATP-dependent RNA helicase DBP9 antibody, ARFGAP1 antibody, Arfgap1 antibody, arfgap1.S antibody, Tb11.01.6060 antibody, arfgap1 antibody, LMJF_32_1230 antibody, MGYG_02419 antibody

Background:

Description: GTPase-activating protein (GAP) for the ADP ribosylation factor 1 (ARF1). Involved in membrane trafficking and /or vesicle transport. Promotes hydrolysis of the ARF1-bound GTP and thus, is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles, a prerequisite for vesicle's fusion with target compartment. Probably regulates ARF1-mediated transport via its interaction with the KDELR proteins and TMED2. Overexpression induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, as when ARF1 is deactivated. Its activity is stimulated by phosphoinosides and inhibited by phosphatidylcholine (By similarity).

Gene: ARFGAP1

Molecular Weight: 60/48 kDa

Gene ID: 55738

UniProt: Q8N6T3

Pathways: p53 Signaling, ER-Nucleus Signaling, Unfolded Protein Response