TDP1 antibody (Middle Region)

Catalog No. ABIN2787112

The Rabbit Polyclonal anti-TDP1 antibody (ABIN2787112) specifically detects TDP1 in WB. The antibody is reactive with Human, Mouse, Rat, Horse, Cow, Guinea Pig, Dog and Rabbit samples.

Quick Overview for TDP1 antibody (Middle Region) (ABIN2787112)

Target: TDP1 (Tyrosyl-DNA Phosphodiesterase 1 (TDP1))

Reactivity: Human, Mouse, Rat, Horse, Cow, Guinea Pig, Dog, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TDP1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-TDP1 Antibody

Binding Specificity: Middle Region

Purpose: Tdp1 Antibody - middle region

Sequence: ETNVYLIGST PGRFQGSHRD NWGHFRLRKL LQAHAPSTPK GECWPIVGQF

Predicted Reactivity: Cow: 92%, Dog: 93%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 86%, Rabbit: 93%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against Tdp1. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide corresponding to a region of Mouse

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP57199-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for TDP1

Target: TDP1 (Tyrosyl-DNA Phosphodiesterase 1 (TDP1))

Alternative Name: Tdp1

Background:

Background Information: Tdp1 is a DNA repair enzyme that can remove a variety of covalent adducts from DNA through hydrolysis of a 3'-phosphodiester bond, giving rise to DNA with a free 3' phosphate. It catalyzes the hydrolysis of dead-end complexes between DNA and the topoisomerase I active site tyrosine residue. It hydrolyzes 3'-phosphoglycolates on protruding 3' ends on DNA double-strand breaks due to DNA damage by radiation and free radicals. It acts on blunt-ended double-strand DNA breaks and on single-stranded DNA. It has low 3'exonuclease activity and can remove a single nucleoside from the 3'end of DNA and RNA molecules with 3'hydroxyl groups. It has no exonuclease activity towards DNA or RNA with a 3'phosphate.

Gene Name: Tyrosyl-DNA phosphodiesterase 1

Alternative Symbols: SC, SCAN1, Gm40556, MLZ-501, AI838772, AW493413, 2810481F14Rik, 4921509N21Rik, E430034L06Rik

Molecular Weight: 64kDa

Gene ID: 104884

NCBI Accession: NP_082630

UniProt: Q8BJ37