SMEK1 antibody (Middle Region)

Catalog No. ABIN2787340

Product Details anti-SMEK1 Antibody

Target: SMEK1 (Serine/threonine-Protein Phosphatase 4 Regulatory Subunit 3A (SMEK1))

Binding Specificity: Middle Region

Reactivity: Mouse, Human, Rat, Zebrafish (Danio rerio), Cow, Dog, Horse, Rabbit, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMEK1 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: YWKALEDVDY VQTFKGLKLR FEQQRERQDN PKLDSMRSIL RNHRYRRDAR

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against SMEK1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human SMEK1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 820 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for SMEK1

Target: SMEK1 (Serine/threonine-Protein Phosphatase 4 Regulatory Subunit 3A (SMEK1))

Alternative Name: SMEK1 (SMEK1 Products)

Synonyms: FLFL1 antibody, KIAA2010 antibody, PP4R3A antibody, smk-1 antibody, smk1 antibody, 1110034C04Rik antibody, BC064465 antibody, mKIAA2010 antibody, RGD1309059 antibody, protein phosphatase 4 regulatory subunit 3A antibody, protein phosphatase 4, regulatory subunit 3A antibody, PPP4R3A antibody, Ppp4r3a antibody

Background: SMEK1 is the regulatory subunit of serine/threonine-protein phosphatase 4.SMEK1 may regulate the activity of PPP4C at centrosomal microtubule organizing centers. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AFX phosphorylated on 'Ser-140' (gamma-H2AFX) generated during DNA replication and required for DNA DSB repair.
Alias Symbols: FLFL1, KIAA2010, MSTP033, smk-1, smk1, PP4R3A
Protein Interaction Partner: HUWE1, UBC, PPP4R2, BRCC3, SF3A1, HNRNPR, TELO2, MTA2, PSMB1, PSMA2, DDB2, CAST, ARCN1, AMPD2, ACACA, CASK, POLR2A, IGBP1, PPP4C, HDAC1, MAPK14,
Protein Size: 820

Molecular Weight: 94 kDa

Gene ID: 55671

NCBI Accession: NM_032560, NP_115949

Pathways: Regulation of Carbohydrate Metabolic Process