RAD1 antibody (Middle Region)

Catalog No. ABIN2787356

The Rabbit Polyclonal anti-RAD1 antibody has been validated for WB. It is suitable to detect RAD1 in samples from Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse and Rabbit.

Quick Overview for RAD1 antibody (Middle Region) (ABIN2787356)

Target: RAD1 (Cell Cycle Checkpoint Protein RAD1 (RAD1))

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAD1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-RAD1 Antibody

Binding Specificity: Middle Region

Sequence: ITMSPDKPYF RLSTFGNAGS SHLDYPKDSD LMEAFHCNQT QVNRYKISLL

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 93%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against RAD1. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human RAD1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 282 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for RAD1

Target: RAD1 (Cell Cycle Checkpoint Protein RAD1 (RAD1))

Alternative Name: RAD1

Background: This gene encodes a component of a heterotrimeric cell cycle checkpoint complex, known as the 9-1-1 complex, that is activated to stop cell cycle progression in response to DNA damage or incomplete DNA replication. The 9-1-1 complex is recruited by RAD17 to affected sites where it may attract specialized DNA polymerases and other DNA repair effectors. Alternatively spliced transcript variants of this gene have been described.
Alias Symbols: HRAD1, REC1
Protein Interaction Partner: ATRX, FEN1, UBC, MSH3, MSH2, MSH6, COPS5, HUS1, ATR, MAP1LC3B, HUS1B, RAD9B, RAD17, POLB, DNAJC7, PARK2, RPA2, RPA1, RAD9A,
Protein Size: 282

Molecular Weight: 32 kDa

Gene ID: 5810

NCBI Accession: NM_002853, NP_002844

UniProt: O60671

Pathways: M Phase