ASAH1 antibody (Middle Region)

Catalog No. ABIN2787376

The Rabbit Polyclonal anti-ASAH1 antibody (ABIN2787376) specifically detects ASAH1 in WB and IHC. The antibody is reactive with Human, Mouse, Rat, Dog, Cow, Guinea Pig, Horse and Pig samples.

Quick Overview for ASAH1 antibody (Middle Region) (ABIN2787376)

Target: ASAH1 (N-Acylsphingosine Amidohydrolase (Acid Ceramidase) 1 (ASAH1))

Reactivity: Human, Mouse, Rat, Dog, Cow, Guinea Pig, Horse, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ASAH1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-ASAH1 Antibody

Binding Specificity: Middle Region

Purpose: ASAH1 Antibody - middle region

Sequence: QSGEGCVITR DRKESLDVYE LDAKQGRWYV VQTNYDRWKH PFFLDDRRTP

Predicted Reactivity: Cow: 85%, Dog: 85%, Guinea Pig: 92%, Horse: 92%, Human: 100%, Mouse: 92%, Pig: 100%, Rat: 92%

Characteristics: This is a rabbit polyclonal antibody against ASAH1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human ASAH1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP57761-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for ASAH1

Target: ASAH1 (N-Acylsphingosine Amidohydrolase (Acid Ceramidase) 1 (ASAH1))

Alternative Name: ASAH1

Background:

Background Information: This gene encodes a heterodimeric protein consisting of a nonglycosylated alpha subunit and a glycosylated beta subunit that is cleaved to the mature enzyme posttranslationally. The encoded protein catalyzes the synthesis and degradation of ceramide into

Gene Name: N-acylsphingosine amidohydrolase (acid ceramidase) 1

Alternative Symbols: AC, PHP, ASAH, PHP32, ACDase, SMAPME

Protein Name: Acid ceramidase

Molecular Weight: 14kDa

Gene ID: 427

NCBI Accession: NP_808592

UniProt: Q13510