PKM2 antibody (Middle Region)

Catalog No. ABIN2787394

Product Details anti-PKM2 Antibody

Target: PKM2 (Pyruvate Kinase M2 (PKM2))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Rabbit, Cow, Dog, Guinea Pig, Horse, Saccharomyces cerevisiae, Sheep, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PKM2 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: HLQLFEELRR LAPITSDPTE ATAVGAVEAS FKCCSGAIIV LTKSGRSAHQ

Predicted Reactivity: Cow: 93%, Dog: 93%, Guinea Pig: 100%, Horse: 93%, Human: 100%, Mouse: 100%, Rabbit: 93%, Rat: 100%, Sheep: 77%, Yeast: 85%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against PKM2. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human PKM2

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 531 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for PKM2

Target: PKM2 (Pyruvate Kinase M2 (PKM2))

Alternative Name: PKM2 (PKM2 Products)

Background: This gene encodes a protein involved in glycolysis. The encoded protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. This protein has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Three alternatively spliced transcript variants encoding two distinct isoforms have been reported.
Alias Symbols: CTHBP, MGC3932, OIP3, PK3, PKM, TCB, THBP1, PKM2
Protein Interaction Partner: LNX1, NXT2, HUWE1, ISG15, UBC, POLE2, FUS, SUMO2, SUMO3, STAU1, MDM2, SUZ12, EZH2, IPO11, TWF2, PDLIM5, NPLOC4, PLIN3, UBA2, EIF4A3, ANP32A, SRP14, RAD23B, PPP1CA, PIN1, CTPS1, CBS, CAPZA2, ACAT2, NLK, FBXO6, YWHAQ, RAF1, IGSF8, HDAC6, MAPK7, NPM1, CDK2,
Protein Size: 531

Molecular Weight: 58 kDa

Gene ID: 5315

NCBI Accession: NM_002654, NP_002645

UniProt: P11980

Pathways: Warburg Effect