APEX1 antibody (N-Term)

Catalog No. ABIN2787518

The Rabbit Polyclonal anti-APEX1 antibody is suitable to detect APEX1 in samples from Human and Pig. It has been validated for WB.

Quick Overview for APEX1 antibody (N-Term) (ABIN2787518)

Target: APEX1 (Apurinic/Apyrimidinic Endonuclease 1 (APEX1))

Reactivity: Human, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This APEX1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-APEX1 Antibody

Binding Specificity: N-Term

Sequence: MPKRGKKGAV AEDGDELRTE PEAKKSKTAA KKNDKEAAGE GPALYEDPPD

Predicted Reactivity: Human: 100%, Pig: 79%

Characteristics: This is a rabbit polyclonal antibody against APEX1. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human APEX1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 318 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for APEX1

Target: APEX1 (Apurinic/Apyrimidinic Endonuclease 1 (APEX1))

Alternative Name: APEX1

Background: Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site. This gene encodes the major AP endonuclease in human cells. Splice variants have been found for this gene, all encode the same protein.
Alias Symbols: APE, APE1, APEN, APEX, APX, HAP1, REF-1, REF1
Protein Interaction Partner: GADD45A, UBC, RPA3, RPA2, RPA1, rev, CCDC124, RIC8A, PABPC1, TWF2, XPOT, HNRNPUL1, ARIH2, PAPSS2, NAE1, PSMG1, TXNRD1, TXN, TCEB1, LGALS1, HNRNPK, CAPNS1, SRPK2, SRPK1, MUTYH, HMGA1, HMGA2, GZMK, GZMA, YBX1, EP300, ASCL2, STAT3, SNRPD1, SFPQ, APP, UBE2I,
Protein Size: 318

Molecular Weight: 35 kDa

Gene ID: 328

NCBI Accession: NM_001641, NP_001632

UniProt: P27695

Pathways: DNA Damage Repair, Chromatin Binding, Cell RedoxHomeostasis, Smooth Muscle Cell Migration, Positive Regulation of Response to DNA Damage Stimulus