MECOM antibody (N-Term)

Catalog No. ABIN2787657

The Rabbit Polyclonal anti-MECOM antibody has been validated for WB. It is suitable to detect MECOM in samples from Human, Mouse, Rat, Dog, Cow, Guinea Pig, Horse, Rabbit and Pig.

Quick Overview for MECOM antibody (N-Term) (ABIN2787657)

Target: MECOM (MDS1 and EVI1 Complex Locus (MECOM))

Reactivity: Human, Mouse, Rat, Dog, Cow, Guinea Pig, Horse, Rabbit, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MECOM antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MECOM Antibody

Binding Specificity: N-Term

Sequence: VKGLSSTEQT NKSQSPLMTH PQILPATQDI LKALSKHPSV GDNKPVELQP

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 86%, Pig: 100%, Rabbit: 100%, Rat: 93%

Characteristics: This is a rabbit polyclonal antibody against EVI1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human EVI1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 1051 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for MECOM

Target: MECOM (MDS1 and EVI1 Complex Locus (MECOM))

Alternative Name: EVI1

Background: EVI1 promotes cell proliferation by interacting with BRG1 and blocking the repression of BRG1 on E2F1 activity.
Alias Symbols: AML1-EVI-1, EVI-1, MDS1-EVI1, MGC163392, PRDM3, EVI1, MDS1
Protein Interaction Partner: PRMT1, PIAS4, SUMO1, SOX2, ELAVL1, SIRT1, KAT2B, SMAD4, SMAD3, SMAD2, SMAD1, EHMT2, SUV39H1, MECOM, HDAC1, HDAC4, CTBP2, CTBP1, CREBBP, MBD3,
Protein Size: 1051

Molecular Weight: 118 kDa

Gene ID: 2122

NCBI Accession: NM_005241, NP_005232

UniProt: Q03112