Caspase 1 antibody (Middle Region)

Catalog No. ABIN2787901

The Rabbit Polyclonal anti-Caspase 1 antibody has been validated for WB. It is suitable to detect Caspase 1 in samples from Human, Horse, Rabbit and Saccharomyces cerevisiae.

Quick Overview for Caspase 1 antibody (Middle Region) (ABIN2787901)

Target: Caspase 1 (CASP1)

Reactivity: Human, Horse, Rabbit, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Caspase 1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Caspase 1 Antibody

Binding Specificity: Middle Region

Sequence: LQTRVLNKEE MEKVKRENAT VMDKTRALID SVIPKGAQAC QICITYICEE

Predicted Reactivity: Horse: 79%, Human: 100%, Rabbit: 79%, Yeast: 100%

Characteristics: This is a rabbit polyclonal antibody against CASP1. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human CASP1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 383 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for Caspase 1

Target: Caspase 1 (CASP1)

Alternative Name: CASP1

Background: This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This gene was identified by its ability to proteolytically cleave and activate the inactive precursor of interleukin-1, a cytokine involved in the processes such as inflammation, septic shock, and wound healing. This gene has been shown to induce cell apoptosis and may function in various developmental stages. Studies of a similar gene in mouse suggest a role in the pathogenesis of Huntington disease. Alternative splicing of this gene results in five transcript variants encoding distinct isoforms.
Alias Symbols: ICE, IL1BC, P45
Protein Interaction Partner: ced-3, ced-4, VAC14, BCL2L1, UBC, XK, PLA2G4B, IL33, IL1B, PARP1, BIRC3, TRAF2, CEBPB, TIRAP, CARD17, CARD16, CARD8, PLA2G4A, CDK11B, NLRP1, ATN1, ATXN3, PYCARD, NLRC4, NOD1, CASP14, RIPK2, PAK1, PARK2, NEDD4, BID, IL18, PSEN2, NFE2L2, AR, BCAP31, MAPT, L
Protein Size: 383

Molecular Weight: 10 kDa

Gene ID: 834

NCBI Accession: NM_001223, NP_001214

UniProt: P29466

Pathways: Apoptosis, Interferon-gamma Pathway, Positive Regulation of Endopeptidase Activity, Inflammasome