Caspase 3 antibody (C-Term)

Catalog No. ABIN2787903

The Rabbit Polyclonal anti-Caspase 3 antibody (ABIN2787903) specifically detects Caspase 3 in WB, IHC and IF. The antibody is reactive with Human, Mouse, Rat, Cow, Dog, Pig, Rabbit, Guinea Pig, Sheep, Horse and Goat samples.

Quick Overview for Caspase 3 antibody (C-Term) (ABIN2787903)

Target: Caspase 3 (CASP3)

Reactivity: Human, Mouse, Rat, Cow, Dog, Pig, Rabbit, Guinea Pig, Sheep, Horse, Goat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Caspase 3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-Caspase 3 Antibody

Binding Specificity: C-Term

Purpose: CASP3 Antibody - C-terminal region

Sequence: NLKYEVRNKN DLTREEIVEL MRDVSKEDHS KRSSFVCVLL SHGEEGIIFG

Predicted Reactivity: Cow: 91%, Dog: 93%, Goat: 86%, Guinea Pig: 86%, Horse: 93%, Human: 100%, Mouse: 86%, Pig: 100%, Rabbit: 100%, Rat: 100%, Sheep: 86%

Characteristics: This is a rabbit polyclonal antibody against CASP3. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human CASP3

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP58987-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for Caspase 3

Target: Caspase 3 (CASP3)

Alternative Name: CASP3

Background:

Background Information: CASP3 is a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein.

Gene Name: Caspase 3, apoptosis-related cysteine peptidase

Alternative Symbols: CPP32, SCA-1, CPP32B

Protein Name: Caspase-3

Molecular Weight: 12kDa

Gene ID: 836

NCBI Accession: NP_116786

UniProt: P42574

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Sensory Perception of Sound, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation