TLR4 antibody (Middle Region)

Catalog No. ABIN2787920

The Rabbit Polyclonal anti-TLR4 antibody has been validated for WB and IHC. It is suitable to detect TLR4 in samples from Human and Pig.

Quick Overview for TLR4 antibody (Middle Region) (ABIN2787920)

Target: TLR4 (Toll-Like Receptor 4 (TLR4))

Reactivity: Human, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TLR4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-TLR4 Antibody

Binding Specificity: Middle Region

Sequence: LGEFRNEGNL EKFDKSALEG LCNLTIEEFR LAYLDYYLDD IIDLFNCLTN

Predicted Reactivity: Human: 100%, Pig: 86%

Characteristics: This is a rabbit polyclonal antibody against TLR4. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human TLR4

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 839 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for TLR4

Target: TLR4 (Toll-Like Receptor 4 (TLR4))

Alternative Name: TLR4

Background: The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This receptor is most abundantly expressed in placenta, and in myelomonocytic subpopulation of the leukocytes. It has been implicated in signal transduction events induced by lipopolysaccharide (LPS) found in most gram-negative bacteria. Mutations in this gene have been associated with differences in LPS responsiveness. Also, several transcript variants of this gene have been found, but the protein coding potential of most of them is uncertain.
Alias Symbols: ARMD10, CD284, TOLL, hToll
Protein Interaction Partner: TNFSF9, SRC, SYK, SMAD3, SERTAD1, BTK, TICAM1, HGS, UBC, HSPA4, TICAM2, STK11IP, TIRAP, TRAF3, TRIM69, IRAK4, TBK1, STUB1, TRAF6, PRKCZ, MYD88, IRAK1, HSPA8, RNF216, CNPY4, SIGIRR, MAPK8IP3, NOX4, TOLLIP, LY96, BCL10, RIPK2, IRAK2, TLR5, DEFB4A, TLR1, HSP
Protein Size: 839

Molecular Weight: 93 kDa

Gene ID: 7099

NCBI Accession: NM_138554, NP_612564

UniProt: O00206

Pathways: TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Toll-Like Receptors Cascades, Inflammasome, S100 Proteins