ATG5 antibody (Middle Region)

Catalog No. ABIN2787927

The Rabbit Polyclonal anti-ATG5 antibody (ABIN2787927) specifically detects ATG5 in WB. The antibody is reactive with Human, Mouse, Rat, Zebrafish (Danio rerio), Cow, Guinea Pig, Horse, Rabbit, Dog and Sheep samples.

Quick Overview for ATG5 antibody (Middle Region) (ABIN2787927)

Target: ATG5 (ATG5 Autophagy Related 5 (ATG5))

Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio), Cow, Guinea Pig, Horse, Rabbit, Dog, Sheep

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATG5 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ATG5 Antibody

Binding Specificity: Middle Region

Purpose: ATG5 Antibody - middle region

Sequence: RFDQFWAINR KLMEYPAEEN GFRYIPFRIY QTTTERPFIQ KLFRPVAADG

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Sheep: 100%, Zebrafish: 79%

Characteristics: This is a rabbit polyclonal antibody against ATG5. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human ATG5

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP59071-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for ATG5

Target: ATG5 (ATG5 Autophagy Related 5 (ATG5))

Alternative Name: ATG5

Background:

Background Information: ATG5 is required for autophagy. It conjugates to ATG12 and associates with isolation membrane to form cup-shaped isolation membrane and autophagosome. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed. ATG5 may play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity.

Gene Name: ATG5 autophagy related 5 homolog (S. cerevisiae)

Alternative Symbols: ASP, APG5, APG5L, hAPG5, SCAR25, APG5-LIKE

Protein Name: Autophagy protein 5

Molecular Weight: 32kDa

Gene ID: 9474

NCBI Accession: NP_004840

UniProt: Q9H1Y0

Pathways: Activation of Innate immune Response, Production of Molecular Mediator of Immune Response, Autophagy