USP8 antibody (C-Term)

Catalog No. ABIN2788008

The Rabbit Polyclonal anti-USP8 antibody (ABIN2788008) specifically detects USP8 in WB and IHC. The antibody is reactive with Human, Mouse, Pig, Rat, Cow, Dog, Horse, Guinea Pig, Rabbit and Zebrafish (Danio rerio) samples.

Quick Overview for USP8 antibody (C-Term) (ABIN2788008)

Target: USP8 (Ubiquitin Specific Peptidase 8 (USP8))

Reactivity: Human, Mouse, Pig, Rat, Cow, Dog, Horse, Guinea Pig, Rabbit, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This USP8 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-USP8 Antibody

Binding Specificity: C-Term

Purpose: USP8 Antibody - C-terminal region

Sequence: FAGYSQQDSQ ELLLFLMDGL HEDLNKADNR KRYKEENNDH LDDFKAAEHA

Predicted Reactivity: Cow: 93%, Dog: 93%, Guinea Pig: 93%, Horse: 93%, Human: 100%, Mouse: 93%, Pig: 100%, Rabbit: 93%, Rat: 93%, Zebrafish: 79%

Characteristics: This is a rabbit polyclonal antibody against USP8. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human USP8

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP59291-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for USP8

Target: USP8 (Ubiquitin Specific Peptidase 8 (USP8))

Alternative Name: USP8

Background:

Background Information: USP8 is a hydrolase that can remove conjugated ubiquitin from proteins and therefore plays an important regulatory role at the level of protein turnover by preventing degradation. USP8 converts both 'Lys-48' an 'Lys-63'-linked ubiquitin chains. USP8 catalytic activity is enhanced in the M phase. USP8 is involved in cell proliferation. USP8 is required to enter into S phase in response to serum stimulation. USP8 may regulate T-cell anergy mediated by RNF128 via the formation of a complex containing RNF128 and OTUB1. USP8 probably regulates the stability of STAM2 and RASGRF1. USP8 regulates endosomal ubiquitin dynamics, cargo sorting, membrane traffic at early endosomes, and maintenance of ESCRT-0 stability. The level of protein ubiquitination on endosomes is essential for maintaining the morphology of the organelle.USP8 deubiquitinates EPS15 and controles tyrosine kinase stability.USP8 removes conjugated ubiquitin from EGFR thus regulating EGFR degradation and downstream MAPK signaling. USP8 is involved in acrosome biogenesis through interaction with the spermatid ESCRT-0 complex and microtubules.

Gene Name: Ubiquitin specific peptidase 8

Alternative Symbols: UBPY, PITA4, SPG59, HumORF8

Protein Name: Ubiquitin carboxyl-terminal hydrolase 8

Molecular Weight: 127kDa

Gene ID: 9101

NCBI Accession: NP_005145

UniProt: P40818