GCH1 antibody (N-Term)

Catalog No. ABIN2788139

The Rabbit Polyclonal anti-GCH1 antibody is suitable to detect GCH1 in samples from Human, Rat, Cow, Dog and Rabbit. It has been validated for WB.

Quick Overview for GCH1 antibody (N-Term) (ABIN2788139)

Target: GCH1 (GTP Cyclohydrolase 1 (GCH1))

Reactivity: Human, Rat, Cow, Dog, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GCH1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-GCH1 Antibody

Binding Specificity: N-Term

Purpose: GCH1 Antibody - N-terminal region

Sequence: PPRPEAKSAQ PADGWKGERP RSEEDNELNL PNLAAAYSSI LSSLGENPQR

Predicted Reactivity: Cow: 100%, Dog: 100%, Human: 100%, Rabbit: 100%, Rat: 86%

Characteristics: This is a rabbit polyclonal antibody against GCH1. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N-terminal region of Human GCH1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP59619-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for GCH1

Target: GCH1 (GTP Cyclohydrolase 1 (GCH1))

Alternative Name: GCH1

Background:

Background Information: This gene encodes a member of the GTP cyclohydrolase family. The encoded protein is the first and rate-limiting enzyme in tetrahydrobiopterin (BH4) biosynthesis, catalyzing the conversion of GTP into 7,8-dihydroneopterin triphosphate. BH4 is an essential cofactor required by aromatic amino acid hydroxylases as well as nitric oxide synthases. Mutations in this gene are associated with malignant hyperphenylalaninemia and dopa-responsive dystonia. Several alternatively spliced transcript variants encoding different isoforms have been described, however, not all variants give rise to a functional enzyme.

Gene Name: GTP cyclohydrolase 1

Alternative Symbols: GCH, DYT5, DYT14, DYT5a, GTPCH1, HPABH4B, GTP-CH-1

Protein Name: GTP cyclohydrolase 1

Molecular Weight: 27kDa

Gene ID: 2643

UniProt: P30793