MRPL49 antibody (N-Term)

Catalog No. ABIN2788326

The Rabbit Polyclonal anti-MRPL49 antibody is suitable to detect MRPL49 in samples from Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse and Rabbit. It has been validated for WB.

Quick Overview for MRPL49 antibody (N-Term) (ABIN2788326)

Target: MRPL49 (Mitochondrial Ribosomal Protein L49 (MRPL49))

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MRPL49 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MRPL49 Antibody

Binding Specificity: N-Term

Purpose: MRPL49 Antibody - N-terminal region

Sequence: ESVDEYQFVE RLLPATRIPD PPKHEHYPTP SGWQPPRDPP PNLPYFVRRS

Predicted Reactivity: Cow: 86%, Dog: 86%, Guinea Pig: 92%, Horse: 92%, Human: 100%, Mouse: 79%, Rabbit: 83%, Rat: 86%

Characteristics: This is a rabbit polyclonal antibody against MRPL49. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human MRPL49

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP60087-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for MRPL49

Target: MRPL49 (Mitochondrial Ribosomal Protein L49 (MRPL49))

Alternative Name: MRPL49

Background:

Background Information: Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75 % protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. This gene and the gene for the HRD1 protein use in their respective 3' UTRs some of the same genomic sequence. Pseudogenes corresponding to this gene are found on chromosomes 5q and 8p.

Gene Name: Mitochondrial ribosomal protein L49

Alternative Symbols: NOF, NOF1, L49mt, C11orf4, MRP-L49

Protein Name: 39S ribosomal protein L49, mitochondrial

Molecular Weight: 19kDa

Gene ID: 740

NCBI Accession: NP_004918

UniProt: Q13405