AKR1C1 antibody (N-Term)

Catalog No. ABIN2788358

The Rabbit Polyclonal anti-AKR1C1 antibody is suitable to detect AKR1C1 in samples from Human, Rat, Cow, Dog, Horse, Rabbit and Pig. It has been validated for WB and IHC.

Quick Overview for AKR1C1 antibody (N-Term) (ABIN2788358)

Target: AKR1C1 (DDH) (Dihydrodiol Dehydrogenase (DDH))

Reactivity: Human, Rat, Cow, Dog, Horse, Rabbit, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AKR1C1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-AKR1C1 Antibody

Binding Specificity: N-Term

Purpose: AKR1C1 Antibody - N-terminal region

Sequence: LERSLKNLQL DYVDLYLIHF PVSVKPGEEV IPKDENGKIL FDTVDLCATW

Predicted Reactivity: Cow: 86%, Dog: 77%, Horse: 77%, Human: 100%, Pig: 77%, Rabbit: 82%, Rat: 82%

Characteristics: This is a rabbit polyclonal antibody against AKR1C1. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human AKR1C1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP60164-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for AKR1C1

Target: AKR1C1 (DDH) (Dihydrodiol Dehydrogenase (DDH))

Alternative Name: AKR1C1

Background:

Background Information: This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme catalyzes the reaction of progesterone to the inactive form 20-alpha-hydroxy-progesterone. This gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14.

Gene Name: Aldo-keto reductase family 1, member C1 (dihydrodiol dehydrogenase 1, 20-alpha (3-alpha)-hydroxysteroid dehydrogenase)

Alternative Symbols: C9, DD1, DDH, DDH1, H-37, HBAB, MBAB, HAKRC, DD1/DD2, 2-ALPHA-HSD, 20-ALPHA-HSD

Protein Name: Aldo-keto reductase family 1 member C1

Molecular Weight: 36kDa

Gene ID: 1645

NCBI Accession: NP_001344

UniProt: Q04828

Pathways: Steroid Hormone Biosynthesis, C21-Steroid Hormone Metabolic Process