VPS25 antibody (C-Term)

Catalog No. ABIN2788482

The Rabbit Polyclonal anti-VPS25 antibody is suitable to detect VPS25 in samples from Human, Mouse, Rat, Cow, Dog and Guinea Pig. It has been validated for WB.

Quick Overview for VPS25 antibody (C-Term) (ABIN2788482)

Target: VPS25 (Vacuolar Protein-Sorting-Associated Protein 25 (VPS25))

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This VPS25 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-VPS25 Antibody

Binding Specificity: C-Term

Purpose: Vps25 Antibody - C-terminal region

Sequence: LYELTSGEDT EEEEFHGLDE ATLLRALQAL QQEHKAEIIT VSDGRGVKFF

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 86%, Human: 100%, Mouse: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against Vps25. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C-terminal region of Rat Vps25

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP60528-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for VPS25

Target: VPS25 (Vacuolar Protein-Sorting-Associated Protein 25 (VPS25))

Alternative Name: Vps25

Background:

Background Information: Vps25 is a component of the ESCRT-II complex (endosomal sorting complex required for transport II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. The ESCRT-II complex may also play a role in transcription regulation, possibly via its interaction with ELL.

Gene Name: vacuolar protein sorting 25 homolog (S. cerevisiae)

Alternative Symbols: -

Protein Name: Vacuolar protein-sorting-associated protein 25

Molecular Weight: 19kDa

Gene ID: 681059

NCBI Accession: NP_001166922

UniProt: P0C0A1